Zhang Wiejie, Huang Jing, Wang Wei, Li Qian, Chen Yao, Feng Weiwei, Zheng Daheng, Zhao Ting, Mao Guanghua, Yang Liuqing, Wu Xiangyang
School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Int J Biol Macromol. 2016 Dec;93(Pt A):448-458. doi: 10.1016/j.ijbiomac.2016.08.079. Epub 2016 Sep 2.
An efficient ultrasonic-cellulase-assisted extraction (UCE) of Cistanche tubulosa polysaccharide (CTP) was established. The response surface methodology based on Box-Behnken Design was employed to further optimize extraction conditions. After quaternary ammonium salt precipitation, the polysaccharide of C. tubulosa was characterized by different techniques. The results showed that a maximum polysaccharide yield of 22.31±0.45% was achieved at a pH of 5.2 for 31.5min at 54.1°C. Compared to hot water extraction, the yield of CTP in UCE and polysaccharide content increased to 44.96% and 70.13±2.19%, respectively. There was no marked difference among polysaccharides extracted using different methods from the infrared spectrum. Ultrasonic-cellulase-assisted extraction polysaccharide showed a fibrous structure from scanning electron microscopy and was composed of rhamnose, mannose, glucose, and galactose in a molar ratio of 2.18:1:28.29:1.43 by gas chromatography. The circular dichroism results indicated that polysaccharides had a maximum positive peak around 210nm with different peak values. The thermogravimetric analysis and differential scanning calorimetry were used to test the thermostability of CTP. Besides, CTP demonstrated appreciable antioxidant potential on antioxidant experiments in vitro. The results suggested that UCE is an effective method for CTP extraction and its polysaccharide showed appreciable antioxidant activity.
建立了一种高效的超声-纤维素酶辅助提取管花肉苁蓉多糖（CTP）的方法。采用基于Box-Behnken设计的响应面法进一步优化提取条件。经季铵盐沉淀后，用不同技术对管花肉苁蓉多糖进行了表征。结果表明，在pH为5.2、54.1℃下提取31.5min时，多糖的最大产率达到22.31±0.45%。与热水提取相比，超声-纤维素酶辅助提取法中CTP的产率和多糖含量分别提高到44.96%和70.13±2.19%。从红外光谱来看，不同方法提取的多糖之间没有明显差异。扫描电子显微镜显示超声-纤维素酶辅助提取的多糖呈纤维状结构，气相色谱分析表明其由鼠李糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为2.18:1:28.29:1.43。圆二色性结果表明，多糖在210nm左右有最大正峰，且峰值不同。采用热重分析和差示扫描量热法测试了CTP的热稳定性。此外，CTP在体外抗氧化实验中表现出明显的抗氧化潜力。结果表明，超声-纤维素酶辅助提取法是提取CTP的有效方法，其多糖具有明显的抗氧化活性。
