Khakimov Kh A, Chikhladze Ts A, Khudoliĭ G A, Akuf'ev A I
Genetika. 1989 Mar;25(3):469-76.
The effect of 5-bromo-2'-deoxyuridine (BUDR) radiosensitizing action on meristematic cells of wheat seedlings and human peripheral blood lymphocytes synchronized with 5-fluoro-2'-deoxyuridine at the G1-S boundary was revealed. This effect was only found in the beginning of the S phase. We show that it was conditioned by incorporation of BUDR into special early replicating DNA sequences which are specific for chromosomal mutagenesis. Using the molecular biology methods non-random distribution of 3H-BUDR in the early replicating wheat DNA sequences with different degree of repeating was found.
揭示了5-溴-2'-脱氧尿苷（BUDR）的放射增敏作用对与5-氟-2'-脱氧尿苷在G1-S边界同步化的小麦幼苗分生细胞和人外周血淋巴细胞的影响。这种效应仅在S期开始时发现。我们表明，它是由BUDR掺入特定的早期复制DNA序列所决定的，这些序列对染色体诱变具有特异性。使用分子生物学方法，发现3H-BUDR在具有不同重复程度的早期复制小麦DNA序列中呈非随机分布。
