Decat G, Léonard A, Myncke H
C R Seances Soc Biol Fil. 1987;181(1):62-9.
Rabbit lymphocytes were treated in Go with therapeutical ultrasound waves (1 W/cm2; 1 MHz; continuous wave mode) alone or followed by an additional exposure to 5-bromo-2'-deoxyuridine (BrdU). Doses in the Fresnel zone ranged from 120 to 960 J/cm2; no refrigeration was provided, and temperature increased from 37 degrees C to 45 degrees C. In the Fraunhofer zone, only an ultrasound dose of 960 J/cm2 was used, and the temperature of the insonated blood sample was maintained at 37 degrees C. For the sequential treatment, BrdU (10 micrograms/ml medium) was added immediately after the ultrasound treatment and was left in contact with the lymphocytes for 48 hours. Ultrasound alone did not induce chromosome aberrations, but ultrasound followed by the BrdU exposure resulted in an increased number of abnormal cells.
兔淋巴细胞处于G0期时，单独接受治疗性超声波（1 W/cm2；1 MHz；连续波模式）处理，或在处理后再额外暴露于5-溴-2'-脱氧尿苷（BrdU）。菲涅耳区内的剂量范围为120至960 J/cm2；未进行制冷，温度从37摄氏度升至45摄氏度。在夫琅禾费区内，仅使用960 J/cm2的超声剂量，且被超声处理的血液样本温度维持在37摄氏度。对于序贯治疗，在超声处理后立即添加BrdU（10微克/毫升培养基），并使其与淋巴细胞接触48小时。单独的超声波未诱导染色体畸变，但在超声波处理后再暴露于BrdU会导致异常细胞数量增加。
