Neoplasma. 2016;63(6):999-1006. doi: 10.4149/neo_2016_620.
To evaluate the diagnostic accuracy of peripheral blood kisspeptin (KISS1) mRNA and plasma cancer antigen 125 (CA125) protein of epithelial ovarian cancer (EOC) in previously pregnant patients, we prospectively enrolled 40 EOC patients as cases and 20 uterine fibroids patients with normal ovary as controls. Levels of peripheral blood KISS1 mRNA and plasma CA125 protein was respectively measured by RT-PCR and electrochemiluminescent method, respectively. Receiver operating characteristic curves with area under curve (AUC) were used to evaluate the diagnostic accuracy. Logistic regression analysis was used to obtain a prediction model for combined diagnosis of KISS1 mRNA and CA125 protein. Both KISS1 mRNA and CA125 protein and had good diagnostic accuracy for EOC, early EOC and advanced EOC (AUC > 0.5, P < 0.05). The CA125 protein had higher diagnostic accuracy than KISS1 mRNA for advanced EOC (P = 0.0009). Moreover, the combination of KISS1 mRNA and CA125 protein had higher diagnostic accuracy for EOC than them alone (P 0.05). Peripheral blood KISS1 mRNA was a novel biomarker for detecting EOC in previously pregnant patients. Combination application of KISS1 mRNA and CA125 protein was recommended for the diagnosis of EOC, but not for advanced and early EOC.
为了评估外周血 kisspeptin（KISS1）mRNA 和上皮性卵巢癌（EOC）患者血浆癌抗原 125（CA125）蛋白的诊断准确性，我们前瞻性纳入 40 例 EOC 患者为病例组，20 例伴有正常卵巢的子宫肌瘤患者为对照组。分别采用 RT-PCR 和电化学发光法检测外周血 KISS1mRNA 和血浆 CA125 蛋白水平。采用受试者工作特征曲线（ROC）下面积（AUC）评价诊断准确性。采用 Logistic 回归分析获得 KISS1mRNA 和 CA125 蛋白联合诊断的预测模型。KISS1mRNA 和 CA125 蛋白对 EOC、早期 EOC 和晚期 EOC 均具有较好的诊断准确性（AUC>0.5，P<0.05）。CA125 蛋白对晚期 EOC 的诊断准确性高于 KISS1mRNA（P=0.0009）。此外，KISS1mRNA 和 CA125 蛋白联合检测对 EOC 的诊断准确性高于单独检测（P<0.05）。外周血 KISS1mRNA 是一种检测既往妊娠患者 EOC 的新型生物标志物。推荐联合应用 KISS1mRNA 和 CA125 蛋白诊断 EOC，但不用于诊断早期和晚期 EOC。
