传统草药配方芍姜龙汤给药大鼠肝脏中细胞色素P450s的表达
Expression of Cytochrome P450s in the Liver of Rats Administered with Socheongryong-tang, a Traditional Herbal Formula.
作者信息
Jin Seong Eun, Ha Hyekyung, Seo Chang-Seob, Shin Hyeun-Kyoo, Jeong Soo-Jin
机构信息
K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
出版信息
Pharmacogn Mag. 2016 Jul-Sep;12(47):211-8. doi: 10.4103/0973-1296.186340.
OBJECTIVE
The purpose of this study was to investigate the potential influences of Socheongryong-tang (SCRT) on the messenger ribonucleic acid (mRNA) and protein expression of cytochrome P450 (CYP450) in vivo.
MATERIALS AND METHODS
SCRT was orally administered to either male or female Sprague-Dawley rats once daily at doses of 0, 1000, 2000, or 5000 mg/kg/day for 13 weeks. The mRNA expression of CYP450s (CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) in liver tissues was measured by reverse transcription polymerase chain reaction. And then, the protein expression of CYP1A1 and CYP2B1/2 in liver tissues was analyzed by the Western blot.
RESULTS
We found no significant influence in the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1 after repeated administration of SCRT for 13 weeks. By contrast, the mRNA and protein expression of hepatic CYP1A1 was increased by repeated SCRT treatment in male rats, but not in female rats. The mRNA and protein expression of hepatic CYP2B1/2 in both genders was increased by administration of SCRT.
CONCLUSION
A caution is needed when SCRT is co-administered with substrates of CYP2B1/2 for clinical usage. In case of male, an attention is also required when SCRT and drugs metabolized by CYP1A1 are taken together. Our findings provide information regarding the safety and effectiveness of SCRT when combined with conventional drugs.
SUMMARY
Oral administration of Socheongryong-tang for 13 weeks did not affect the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1In male rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP1A1 and CYP2B1/2In female rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP2B1/2. Abbreviations used: SCRT: Socheongryong-tang, CYP450: Cytochrome P450, HPLC: High performance liquid chromatography,
RT-PCR: Reverse transcription polymerase chain reaction.
目的
本研究旨在探讨柴胡清肝汤（SCRT）对体内细胞色素P450（CYP450）信使核糖核酸（mRNA）和蛋白表达的潜在影响。
材料与方法
将雄性或雌性Sprague-Dawley大鼠每日口服给予SCRT，剂量分别为0、1000、2000或5000mg/kg/天，持续13周。通过逆转录聚合酶链反应测定肝组织中CYP450s（CYP1A1、1A2、2B1/2、2C11、2E1、3A1、3A2和4A1）的mRNA表达。然后，通过蛋白质印迹法分析肝组织中CYP1A1和CYP2B1/2的蛋白表达。
结果
我们发现，连续13周给予SCRT后，肝CYP1A2、2C11、2E1、3A1、3A2和4A1的mRNA表达没有显著影响。相比之下，雄性大鼠反复给予SCRT可增加肝CYP1A1的mRNA和蛋白表达，而雌性大鼠则无此现象。给予SCRT可增加两性肝CYP2B1/2的mRNA和蛋白表达。
结论
临床使用SCRT与CYP2B1/2底物合用时需谨慎。对于男性，SCRT与经CYP1A1代谢的药物合用时也需注意。我们的研究结果提供了SCRT与传统药物联合使用时的安全性和有效性信息。
总结
口服柴胡清肝汤13周不影响肝CYP1A2、2C11、2E1、3A1、3A2和4A1的mRNA表达。在雄性大鼠中，口服柴胡清肝汤13周可诱导肝CYP1A1和CYP2B1/2的mRNA和蛋白表达。在雌性大鼠中，口服柴胡清肝汤13周可诱导肝CYP2B1/2的mRNA和蛋白表达。使用的缩写：SCRT：柴胡清肝汤，CYP450：细胞色素P450，HPLC：高效液相色谱，RT-PCR：逆转录聚合酶链反应。
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