Sato Hiroki, Suzuki Jun-Ichi, Aoyama Norio, Watanabe Ryo, Kaneko Makoto, Shiheido Yuka, Yoshida Asuka, Wakayama Kouji, Kumagai Hidetoshi, Ikeda Yuichi, Akazawa Hiroshi, Komuro Issei, Isobe Mitsuaki, Izumi Yuichi
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Departments of Advanced Clinical Science and Therapeutics, The University of Tokyo, Tokyo, Japan.
Hypertens Res. 2017 Jan;40(1):35-40. doi: 10.1038/hr.2016.114. Epub 2016 Sep 8.
Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23% larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.
心力衰竭是一种由多种病症引发的严重疾病，包括肥厚型心肌病。尽管许多报告表明牙周疾病与心血管疾病之间存在关联，但其机制尚未阐明。本研究的目的是阐明牙周疾病与心脏病之间的关系，尤其是在心肌肥大方面。我们使用C57BL/6J小鼠并植入了两种皮下腔室。首先，我们在小鼠背部皮下植入一个螺旋形腔室。将主要的牙周病原体牙龈卟啉单胞菌（P.g.）注入该腔室。然后，植入一个渗透泵以输注异丙肾上腺素。在输注ISO四周后，我们进行了超声心动图检查并采集了心脏和血液。我们使用ELISA测量血清抗P.g.-IgG水平。使用PCR测量几种因子的mRNA水平。我们发现与ISO(+)/P.g.(-)小鼠相比，ISO(+)/P.g.(+)小鼠的心肌细胞肥大更明显。ISO(+)/P.g.(+)小鼠中随机选择的心肌细胞的总面积比ISO(+)/P.g.(-)小鼠大23%。与对照组相比，我们在ISO(+)/P.g.(-)小鼠中检测到Toll样受体2和NADPH氧化酶4的mRNA表达水平更高。我们揭示了一种牙周病原体通过氧化应激影响ISO诱导的心肌肥大。
