The Department of Gastroenterology, the Second Hospital, Zhengzhou University, Zhengzhou, China.
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
Sci Rep. 2016 Sep 8;6:32765. doi: 10.1038/srep32765.
The pathogenesis of intestinal chronic inflammation is unclear. Food allergy plays an important role in the induction of intestinal inflammation. This study aims to test a hypothesis that food allergy initiates colitis. In this study, BALB/c mice were sensitized to a common food allergen, ovalbumin (OVA) with cholera toxin (CT) as an adjuvant. The colon epithelial barrier function was assessed with Ussing chamber technique. Expression of T cell immunoglobulin mucin domain molecule-4 (TIM4) in dendritic cells was evaluated by flow cytometry, RT-PCR and Western blotting. The results showed that allergen-related colitis was induced in mice as shown by heavy infiltration of inflammatory cells in the colon mucosa, loss of body weight of mice, increases in myeloperoxidase, tumor necrosis factor-α, interleukin-4, OVA-specific IgE in the colon tissue. The colon epithelial barrier function was markedly compromised in colitis group mice, which was mimicked by exposure the colon mucosa to CT in Ussing chamber. High frequency of TIM4(+) dendritic cells was detected in the colon mucosa of colitis mice. Exposure of dendritic cells to CT markedly increased the expression of TIM4. We conclude that IBD-like inflammation can be induced in the mouse colon by the food allergen-related immune response.
肠道慢性炎症的发病机制尚不清楚。食物过敏在诱导肠道炎症中起重要作用。本研究旨在验证食物过敏引发结肠炎的假设。在这项研究中，BALB/c 小鼠用霍乱毒素 (CT) 作为佐剂对常见食物过敏原卵清蛋白 (OVA) 进行致敏。通过 Ussing 室技术评估结肠上皮屏障功能。通过流式细胞术、RT-PCR 和 Western blot 评估树突状细胞中 T 细胞免疫球蛋白粘蛋白结构域分子-4 (TIM4) 的表达。结果表明，过敏原相关结肠炎在小鼠中诱导，表现为结肠黏膜炎症细胞重度浸润、小鼠体重减轻、髓过氧化物酶、肿瘤坏死因子-α、白细胞介素-4、结肠组织中 OVA 特异性 IgE 增加。结肠炎组小鼠的结肠上皮屏障功能明显受损，在 Ussing 室中用 CT 暴露结肠黏膜可模拟这种情况。在结肠炎小鼠的结肠黏膜中检测到高频 TIM4(+)树突状细胞。树突状细胞暴露于 CT 可显著增加 TIM4 的表达。我们得出结论，食物过敏原相关免疫反应可在小鼠结肠中诱导类似 IBD 的炎症。
