Kim S Y, Byrn R, Groopman J, Baltimore D
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.
J Virol. 1989 Sep;63(9):3708-13. doi: 10.1128/JVI.63.9.3708-3713.1989.
The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-length viral linear DNA is first detectable by 4 h postinfection. During a one-step growth of HIV, amounts of viral DNA gradually increase until 8 to 12 h postinfection and then decrease. The copy number of unintegrated viral DNA is not extraordinarily high even at its peak. Most strikingly, there is a temporal program of RNA accumulation: the earliest RNA is greatly enriched in the 2-kilobase subgenomic mRNA species, while the level of 9.2-kilobase RNA which is both genomic RNA and mRNA remains low until after 24 h of infection. Virus production begins at about 24 h postinfection. Thus, viral DNA synthesis is as rapid as for other retroviruses, but viral RNA synthesis involves temporal alteration in the species that accumulate, presumably as a consequence of viral regulatory genes.
逆转录病毒DNA和RNA合成的动力学是理解病毒生长的关键参数，对于人类免疫缺陷病毒（HIV）尤为重要，因为HIV编码了多个自身的调控基因。我们已经在人CD4+淋巴细胞系H9细胞中建立了HIV的单周期生长条件。感染后4小时可首次检测到全长病毒线性DNA。在HIV的一步生长过程中，病毒DNA的量逐渐增加，直到感染后8至12小时，然后减少。即使在峰值时，未整合病毒DNA的拷贝数也不是特别高。最引人注目的是，存在一个RNA积累的时间程序：最早的RNA在2千碱基亚基因组mRNA种类中大量富集，而作为基因组RNA和mRNA的9.2千碱基RNA的水平在感染24小时后才开始升高。病毒产生大约在感染后24小时开始。因此，病毒DNA合成与其他逆转录病毒一样迅速，但病毒RNA合成涉及积累种类的时间变化，这可能是病毒调控基因作用的结果。
