增强的 Rho 激酶活性：2 型糖尿病中的病理生理相关性。

Enhanced Rho-kinase activity: Pathophysiological relevance in type 2 diabetes.

机构信息

Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Clin Chim Acta. 2016 Nov 1;462:107-110. doi: 10.1016/j.cca.2016.09.003. Epub 2016 Sep 8.

DOI:10.1016/j.cca.2016.09.003

PMID:27616626

Abstract

BACKGROUND

Accumulating evidence indicates that Rho-associated kinase (ROCK) has been involved in the pathogenesis of insulin resistance and diabetes. However, little clinical evidence for ROCK activity in diabetic patients is available. We determined whether ROCK activity is systemically enhanced in type 2 diabetic patients and associated with other components of diabetes.

METHODS

Seventy-eight volunteers, including 41 type 2 diabetic patients and 37 control subjects, were participated in this study. Fasting blood samples were collected to measure ROCK activity in circulating leukocyte, determined by the ratio of phosphorylation/total myosin-binding subunit (MBS), a direct downstream target of ROCK.

RESULTS

Compared with the control subjects, ROCK activity was significantly increased in type 2 diabetic patients (phosphorylation/total MBS ratio 0.80±0.10 vs. 0.72±0.08, P<0.01). An independent positive correlation was found between ROCK activity and HbA concentration in type 2 diabetic patients but not in control subjects (r=0.40, P=0.01). In multiple regression analysis, ROCK activity remains associated significantly in a positive manner with HbA concentration in type 2 diabetes (β=0.03, P=0.04).

CONCLUSIONS

These findings demonstrated that ROCK activity is significantly increased in type 2 diabetic patients and enhanced ROCK activity may reflect the progression of disease.

摘要

背景

越来越多的证据表明 Rho 相关激酶（ROCK）参与了胰岛素抵抗和糖尿病的发病机制。然而，目前关于糖尿病患者 ROCK 活性的临床证据还很少。我们旨在确定 ROCK 活性是否在 2 型糖尿病患者中全身增强，并与糖尿病的其他成分相关。

方法

本研究共纳入 78 名志愿者，包括 41 名 2 型糖尿病患者和 37 名对照者。采集空腹血样以测量循环白细胞中的 ROCK 活性，通过 ROCK 的直接下游靶标肌球蛋白结合亚基（MBS）的磷酸化/总 MBS 比值来确定。

结果

与对照组相比，2 型糖尿病患者的 ROCK 活性显著升高（磷酸化/总 MBS 比值 0.80±0.10 比 0.72±0.08，P<0.01）。在 2 型糖尿病患者中，ROCK 活性与 HbA 浓度呈独立的正相关，但在对照组中无此相关性（r=0.40，P=0.01）。在多元回归分析中，ROCK 活性与 2 型糖尿病患者的 HbA 浓度仍呈正相关（β=0.03，P=0.04）。