Kılıç Raşit, Bayraktar Aslhan Cavunt, Bayraktar Serdar, Kurt Ali, Kavutçu Mustafa
*Department of Ophthalmology, Faculty of Medicine, Ahi Evran University, Kırşehir, Turkey;†Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey; and‡Ulucanlar Eye Training and Research Hospital, Ankara, Turkey.
Cornea. 2016 Dec;35(12):1512-1515. doi: 10.1097/ICO.0000000000001018.
The aim of this study was to evaluate the relationship between antioxidant superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA) as a lipid peroxidation marker, and some trace elements such as zinc (Zn) and copper (Cu) levels in patients with keratoconus.
A total of 58 patients with keratoconus and 53 control subjects with similar age and sex were evaluated in this study. The modified Krumeich keratoconus classification was used to divide the patients into 4 stages. Serum SOD activity, MDA, and zinc and copper levels were compared between the patient and control groups.
The median serum SOD activity, MDA, and Zn and Cu levels were 27.2 (42.4-13.7) U/mL, 10.2 (11.9-8.5) nmol/mL, 87.9 (104.6-76.5) μmol/L, and 103.2 (117.9-90.3) μmol/L in the keratoconus group and 26.2 (32.5-14.4) U/mL, 8.8 (11.4-7.1) nmol/mL, 100.5 (121.1-81.8) μmol/L, and 98.4 (120.3-83.4) μmol/L in the control group, respectively. There was a statistically significant difference between the MDA and Zn levels of the keratoconus group and control subjects but not between the respective SOD activities or Cu levels (P = 0.016, P = 0.031, P = 0.440, and P = 0.376, respectively). We found no significant difference between the keratoconus group stages for serum SOD activity, serum MDA, and Zn and Cu levels (P > 0.05), and there was also no significant correlation between the keratoconus group stages and serum SOD activity, serum MDA, and Zn and Cu levels (P > 0.05).
There is imbalance in the systemic oxidant/antioxidant status where Zn deficiency also plays a role in patients with keratoconus.
本研究旨在评估圆锥角膜患者抗氧化超氧化物歧化酶（SOD）活性、作为脂质过氧化标志物的丙二醛（MDA）以及锌（Zn）和铜（Cu）等一些微量元素水平之间的关系。
本研究共评估了58例圆锥角膜患者和53例年龄及性别相仿的对照者。采用改良的克鲁梅希圆锥角膜分类法将患者分为4个阶段。比较患者组和对照组的血清SOD活性、MDA以及锌和铜水平。
圆锥角膜组血清SOD活性、MDA以及锌和铜水平的中位数分别为27.2（42.4 - 13.7）U/mL、10.2（11.9 - 8.5）nmol/mL、87.9（104.6 - 76.5）μmol/L和103.2（117.9 - 90.3）μmol/L，对照组分别为26.2（32.5 - 14.4）U/mL、8.8（11.4 - 7.1）nmol/mL、100.5（121.1 - 81.8）μmol/L和98.4（120.3 - 83.4）μmol/L。圆锥角膜组与对照者的MDA和锌水平存在统计学显著差异，但各自的SOD活性或铜水平之间无差异（P分别为0.016、0.031、0.440和0.376）。我们发现圆锥角膜组各阶段的血清SOD活性、血清MDA以及锌和铜水平之间无显著差异（P > 0.05），圆锥角膜组各阶段与血清SOD活性、血清MDA以及锌和铜水平之间也无显著相关性（P > 0.05）。
圆锥角膜患者存在全身氧化/抗氧化状态失衡，其中锌缺乏也起一定作用。
