Qin Shenyue, Madhavan Mayur, Waclaw Ronald R, Nakafuku Masato, Campbell Kenneth
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
Molecular and Developmental Biology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
Genesis. 2016 Oct;54(10):542-549. doi: 10.1002/dvg.22980. Epub 2016 Sep 29.
In this study, we generated a transgenic mouse line driving Cre and EGFP expression with two putative cis-regulatory modules (CRMs) (i.e., hs687 and hs678) upstream of the homeobox gene Gsx2 (formerly Gsh2), a critical gene for establishing lateral ganglionic eminence (LGE) identity. The combination of these two CRMs drives transgene expression within the endogenous Gsx2 expression domains along the anterior-posterior neuraxis. By crossing this transgenic line with the Rosa (Ai14) reporter mouse line, we observed a unique recombination pattern in the lateral ventral telencephalon, namely the LGE and the dorsal half of the medial GE (MGE), but not in the septum. We found robust recombination in many cell types derived from these embryonic regions, including olfactory bulb and amygdala interneurons and striatal projection neurons from the LGE, as well as cortical interneurons from the MGE and caudal GE (CGE). In summary, this transgenic mouse line represents a new tool for genetic manipulation in the LGE/CGE and the dorsal half of MGE.
在本研究中，我们构建了一个转基因小鼠品系，该品系利用同源框基因Gsx2（原Gsh2）上游的两个假定顺式调控模块（CRMs）（即hs687和hs678）驱动Cre和EGFP表达，Gsx2是建立外侧神经节隆起（LGE）特征的关键基因。这两个CRMs的组合驱动转基因在沿前后神经轴的内源性Gsx2表达域内表达。通过将该转基因品系与Rosa（Ai14）报告基因小鼠品系杂交，我们在外侧腹侧端脑观察到一种独特的重组模式，即LGE和内侧神经节隆起（MGE）的背侧半部，但在隔膜中未观察到。我们在源自这些胚胎区域的许多细胞类型中发现了强烈的重组，包括嗅球和杏仁核中间神经元以及来自LGE的纹状体投射神经元，以及来自MGE和尾侧神经节隆起（CGE）的皮质中间神经元。总之，这个转基因小鼠品系代表了一种用于在LGE/CGE和MGE背侧半部进行基因操作的新工具。
