Bridi Henrique, Beckenkamp Aline, Ccana-Ccapatinta Gari Vidal, de Loreto Bordignon Sérgio Augusto, Buffon Andréia, von Poser Gilsane Lino
Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Programa de Pós Graduação em Avaliação de Impactos Ambientais, UNILASALLE, Av. Victor Barreto, 2288, Canoas, 92010000, Brazil.
Phytother Res. 2017 Jan;31(1):62-68. doi: 10.1002/ptr.5727. Epub 2016 Sep 13.
In this study, a phytochemical and biological investigation on five South Brazilian Hypericum species (Hypericum caprifoliatum, Hypericum carinatum, Hypericum connatum, Hypericum myrianthum, and Hypericum polyanthemum) was carried out. The phloroglucinol-enriched fractions (PEF) of the flowering aerial parts were analyzed by high-performance liquid chromatography for the content of uliginosin A (1), japonicin A (2), uliginosin B (3), hyperbrasilol B (4), and the three benzopyrans, that is, 6-isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran (HP1) (5), 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran (HP2) (6), and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) (7). After chemical characterization, the PEF were assayed for cell proliferation on human keratinocyte cell line by MTT. The H. carinatum and H. polyanthemum PEF demonstrated better results with an increase in cell proliferation (138.7% and 120.6%, respectively). The cell counting and Ki-67 assay with H. carinatum PEF confirmed the MTT results. The cell cycle distribution indicates an increase in the cells at S and G2/M phases, which is indicative of proliferation induction. In summary, the results indicate an induction of HaCaT proliferation by the treatment with H. carinatum PEF (at 10 and 15 µg/mL), suggesting a possible use as wound healing agent. Copyright © 2016 John Wiley & Sons, Ltd.
在本研究中，对巴西南部的五种金丝桃属植物（荚蒾叶金丝桃、龙骨金丝桃、合生金丝桃、多花金丝桃和多蕊金丝桃）进行了植物化学和生物学研究。采用高效液相色谱法分析了开花地上部分富含间苯三酚的馏分（PEF）中uliginosin A（1）、日本辛素A（2）、uliginosin B（3）、hyperbrasilol B（4）以及三种苯并吡喃类化合物，即6 - 异丁酰基 - 5,7 - 二甲氧基 - 2,2 - 二甲基 - 苯并吡喃（HP1）（5）、7 - 羟基 - 6 - 异丁酰基 - 5 - 甲氧基 - 2,2 - 二甲基 - 苯并吡喃（HP2）（6）和5 - 羟基 - 6 - 异丁酰基 - 7 - 甲氧基 - 2,2 - 二甲基 - 苯并吡喃（HP3）（7）的含量。化学表征后，通过MTT法检测PEF对人角质形成细胞系细胞增殖的影响。龙骨金丝桃和多蕊金丝桃的PEF在细胞增殖方面表现出更好的结果（分别增加了138.7%和120.6%）。用龙骨金丝桃PEF进行的细胞计数和Ki - 67检测证实了MTT结果。细胞周期分布表明S期和G2/M期的细胞增加，这表明有增殖诱导作用。总之，结果表明用龙骨金丝桃PEF（10和15μg/mL）处理可诱导HaCaT细胞增殖，提示其可能用作伤口愈合剂。版权所有© 2016约翰威立父子有限公司。
