Programa de Pós Graduação em Ciências Farmacêuticas, UFRGS. Av. Ipiranga, 2752, 90610.000 Porto Alegre, RS, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):83-7. doi: 10.1016/j.jchromb.2009.11.012.
The aerial parts of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferae) were successively extracted with supercritical carbon dioxide (SC CO(2)) under pressures of 90, 120, 150 and 200 bar at different temperatures (40, 50 and 60 degrees C), and compared with the n-hexane extract obtained by ultrasound-assisted extraction. The samples obtained were examined regarding extraction yield and HPLC quantification of the main secondary metabolites, the benzopyrans HP1 (6-isobutyryl-5,7-dimethoxy-2,2-dimethylbenzopyran), HP2 (7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran) and HP3 (5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl) and the phloroglucinol derivative, uliginosin B. SFE presented higher selectivity than the n-hexane maceration, and the best condition to extract the target metabolites has been determined to be at 50 degrees C and for the high molecular-weight compound, uliginosin B, higher pressures were required.
超基性多腺忍冬（藤黄科）的地上部分依次用超临界二氧化碳（SC CO(2)）在 90、120、150 和 200 巴的压力下在不同温度（40、50 和 60 摄氏度）下提取，并与超声辅助提取得到的正己烷提取物进行比较。对所得样品进行了提取产率和主要次生代谢产物的 HPLC 定量分析，所述次生代谢产物为苯并吡喃 HP1（6-异丁酰基-5,7-二甲氧基-2,2-二甲基苯并吡喃）、HP2（7-羟基-6-异丁酰基-5-甲氧基-2,2-二甲基苯并吡喃）和 HP3（5-羟基-6-异丁酰基-7-甲氧基-2,2-二甲基）和间苯三酚衍生物，uliginosin B。SFE 比正己烷浸渍具有更高的选择性，并且已经确定提取目标代谢物的最佳条件为 50°C，对于高分子量化合物 uliginosin B，需要更高的压力。
