Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Sci Rep. 2016 Sep 13;6:32889. doi: 10.1038/srep32889.
Crohn's disease (CD) and ulcerative colitis (UC) have different immunological mechanisms, while both of them are potential targets of vitamin D treatment. In this study, we have tried to address the role of vitamin D in CD and UC using two mouse models. Mice of C57B6L were given vitamin D before the induction of colitis. Our results showed that vitamin D attenuated 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis but not oxazolone-induced colitis. Vitamin D could preserve the local histology, alleviate inflammation, suppress apoptosis, maintain tight junction function and decrease permeability. Interestingly, it has more of an effect on local structure preservation and inflammation inhibition in CD than in UC mice. Vitamin D blocked the increase of helper T-cell type 1 (Th1)- and helper T-cell type 17 (Th17)-related cytokines in TNBS-induced colitis. But the increase of helper T-cell type 2 (Th2)- and regulatory T cells (Treg)-related cytokines was augmented at the same time in oxazolone-induced colitis which counteracted each other. Our study helps elucidate the differential protective effects of vitamin D on CD and UC patients, as reported in literature.
克罗恩病（CD）和溃疡性结肠炎（UC）具有不同的免疫机制，而它们都是维生素 D 治疗的潜在靶点。在这项研究中，我们使用两种小鼠模型来探讨维生素 D 在 CD 和 UC 中的作用。在结肠炎诱导前，给予 C57B6L 小鼠维生素 D。我们的结果表明，维生素 D 可减轻 2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎，但不能减轻氧化偶氮甲烷诱导的结肠炎。维生素 D 可保持局部组织学，减轻炎症，抑制细胞凋亡，维持紧密连接功能并降低通透性。有趣的是，维生素 D 对 CD 小鼠的局部结构保存和炎症抑制作用比对 UC 小鼠更明显。维生素 D 可阻断 TNBS 诱导的结肠炎中辅助性 T 细胞 1（Th1）和辅助性 T 细胞 17（Th17）相关细胞因子的增加。但是，在氧化偶氮甲烷诱导的结肠炎中，辅助性 T 细胞 2（Th2）和调节性 T 细胞（Treg）相关细胞因子的增加同时增强，相互抵消。我们的研究有助于阐明文献报道的维生素 D 对 CD 和 UC 患者的差异保护作用。
