Water Utilisation and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.
Water Utilisation and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.
J Hazard Mater. 2017 Jan 5;321:218-227. doi: 10.1016/j.jhazmat.2016.08.035. Epub 2016 Aug 20.
Effect of biosurfactant on biodegradation of pyrene was studied using a microbial consortium predominantly composed of Pseudomonas viridiflava (49.5%) and Pseudomonas nitroreducens (32.5%) in a batch experiment containing lipopeptidic biosurfactant, produced by Paenibacillus dendritiformis CN5 strain, and mineral salt medium. The results showed that the lipopeptide at 600 and 300mgL enhanced pyrene degradation to 83.5% and 67% respectively in 24days compared to 16% degradation in its absence. However degradation of pyrene was reduced to 57% as the lipopeptide supplementation was raised to 900mgL. This demonstrates that the biodegradation of pyrene was found to increase with an increase in the lipopeptide concentration up to a threshold level. The experimental data were fitted to the logistic kinetic model which provided best fit with a coefficient of determination (R) values≥0.97. Maximum specific growth rate, μ of 0.97 and 0.69d were achieved in the 600 and 300mgL lipopeptide amendments in comparison to 0.54d in the unamended one. The carrying capacity, X increased 4.4-fold in 600mgL lipopeptide supplemented samples in comparison to its absence. Generally the lipopeptide showed potential application in improving bioremediation of polycyclic aromatic hydrocarbons contaminated environmental media.
生物表面活性剂对芘生物降解的影响研究采用微生物联合体主要由铜绿假单胞菌（49.5%）和假单胞菌（32.5%）在含有脂肽生物表面活性剂，生产的地衣芽孢杆菌 dendritiformis 株，和无机盐培养基的批处理实验。结果表明，脂肽在 600 和 300mgL 分别提高芘的降解 83.5%和 67%，在 24 天内相比，16%的降解。然而，降解的芘减少到 57%，作为脂肽补充提高到 900mgL。这表明，生物降解的芘被发现增加与增加脂肽浓度达到一个阈值水平。实验数据拟合逻辑斯蒂动力学模型提供了最佳的拟合与决定系数（R）值≥0.97。最大比增长速率，μ的 0.97 和 0.69d 实现了在 600 和 300mgL 脂肽修正案相比，0.54d 在未经修正的。承载能力，X 增加了 4.4 倍，在 600mgL 脂肽补充样本相比，其缺席。一般来说，脂肽显示出潜在的应用，提高多环芳烃污染环境介质的生物修复。
