Qiu Yuanyuan, Lee Bonita E, Ruecker Norma J, Neumann Norman, Ashbolt Nicholas, Pang Xiaoli
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada.
Department of Pediatrics, University of Alberta, Edmonton, Canada.
J Virol Methods. 2016 Nov;237:150-153. doi: 10.1016/j.jviromet.2016.09.010. Epub 2016 Sep 11.
A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 100mL wastewater samples through direct centrifugation using the Centricon Plus-70 ultrafilter. Seven viruses, including norovirus, rotavirus, sapovirus, astrovirus, enterovirus, adenovirus and JC virus, were tested using real-time quantitative PCR (rt-qPCR) and cell culture. All of the viruses were detected in pre- and post-UV samples by rt-qPCR, with rotavirus the most numerous (6.6 log GE copies/L). Infectious viruses, by cell culture, were found in all tested pre-UV samples but only in one post-UV sample. The results were comparable and consistent to that obtained using virus adsorption-elution method, indicating that the centrifugal ultrafiltration method is adequate to retain the viruses and maintain their infectivity during processing. As a simple, rapid and cost-effective method to screen wastewater viruses, this one-step centrifugal ultrafiltration method may serve as an effective approach to assess virus removal and gain knowledge of human virus activity during wastewater treatment.
开发了一种一步离心超滤方法，以加强对废水中人类肠道病毒和共现病毒的快速检测。在加拿大卡尔加里的两个全规模三级城市污水处理厂，在紫外线处理前后采集了样本。使用Centricon Plus - 70超滤器通过直接离心从100mL废水样本中浓缩病毒。使用实时定量PCR（rt-qPCR）和细胞培养对包括诺如病毒、轮状病毒、札幌病毒、星状病毒、肠道病毒、腺病毒和JC病毒在内的七种病毒进行了检测。通过rt-qPCR在紫外线处理前后的样本中均检测到了所有病毒，其中轮状病毒数量最多（6.6 log GE拷贝/升）。通过细胞培养，在所有测试的紫外线处理前样本中均发现了感染性病毒，但仅在一个紫外线处理后样本中发现。结果与使用病毒吸附 - 洗脱法获得的结果具有可比性和一致性，表明离心超滤法足以在处理过程中保留病毒并保持其感染性。作为一种筛选废水病毒的简单、快速且经济高效的方法，这种一步离心超滤法可作为评估病毒去除情况以及了解废水处理过程中人类病毒活性的有效方法。
