Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, Canada.
Sections of Pediatric Emergency Medicine and Gastroenterology, Departments of Pediatrics and Emergency Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T3B 6A8, Canada.
Viruses. 2023 Jul 13;15(7):1541. doi: 10.3390/v15071541.
Worldwide, acute gastroenteritis (AGE) is a major cause of morbidity and mortality in children under 5 years of age. Viruses, including norovirus, rotavirus, and enteric adenovirus, are the leading causes of pediatric AGE. In this prospective cohort study, we investigated the viral load and duration of shedding of norovirus, rotavirus, and adenovirus in stool samples collected from 173 children (median age: 15 months) with AGE who presented to emergency departments (EDs) across Canada on Day 0 (day of enrollment), and 5 and 28 days after enrollment. Quantitative RT-qPCR was performed to assess the viral load. On Day 0, norovirus viral load was significantly lower compared to that of rotavirus and adenovirus ( < 0.001). However, on Days 5 and 28, the viral load of norovirus was higher than that of adenovirus and rotavirus ( < 0.05). On Day 28, norovirus was detected in 70% (35/50) of children who submitted stool specimens, while rotavirus and adenovirus were detected in 52.4% (11/24) and 13.6% (3/22) of children ( < 0.001), respectively. Overall, in stool samples of children with AGE who presented to EDs, rotavirus and adenovirus had higher viral loads at presentation compared to norovirus; however, norovirus was shed in stool for the longest duration.
在全球范围内，急性胃肠炎（AGE）是 5 岁以下儿童发病率和死亡率的主要原因。病毒，包括诺如病毒、轮状病毒和肠腺病毒，是导致儿科 AGE 的主要原因。在这项前瞻性队列研究中，我们调查了 173 名患有 AGE 的儿童（中位年龄：15 个月）粪便样本中诺如病毒、轮状病毒和腺病毒的病毒载量和脱落持续时间，这些儿童在加拿大各地的急诊室（ED）于第 0 天（入组日）、入组后第 5 天和第 28 天采集了粪便样本。定量 RT-qPCR 用于评估病毒载量。第 0 天，诺如病毒的病毒载量明显低于轮状病毒和腺病毒（<0.001）。然而，在第 5 天和第 28 天，诺如病毒的病毒载量高于腺病毒和轮状病毒（<0.05）。在第 28 天，提交粪便样本的 50 名儿童中有 70%（35/50）检测到诺如病毒，而轮状病毒和腺病毒在 24 名儿童中的检出率分别为 52.4%（11/24）和 13.6%（3/22）（<0.001）。总体而言，在急诊科就诊的患有 AGE 的儿童的粪便样本中，轮状病毒和腺病毒的病毒载量在就诊时高于诺如病毒；然而，诺如病毒在粪便中的排出时间最长。
