Campos C T, Michalek V N, Matts J P, Buchwald H
Department of Surgery, University of Minnesota Health Sciences Center, Minneapolis 55455.
Surgery. 1989 Aug;106(2):177-84.
Dietary marine oil supplements may protect against atherosclerosis, although their influence on plasma lipids, in vivo cholesterol metabolism, and aortic cholesterol accumulation remains uncertain. The effects of daily administration of marine oil--delivering 100 mg of eicosapentaenoic acid, 59 mg of docosahexaenoic acid, and 221 mg of omega-3 fatty acids per kilogram--were assessed in 33 New Zealand white rabbits. Six animals (group I) were immediately killed. In the remaining animals stable hypercholesterolemia was induced with a 0.25% cholesterol-enriched diet. After 7 weeks on this diet, six animals were killed (group II). Total plasma cholesterol had increased significantly (982 +/- 119 mg/dl vs. 55.6 +/- 7.1 mg/dl, mean +/- SEM, p less than 0.001). The remaining animals randomly received a tap-water placebo (group III, n = 12) or marine oil (group IV, n = 9) daily. After 3 months, total plasma cholesterol was similar (p = NS) among group II (982 +/- 119 mg/dl), group III (965 +/- 54 mg/dl), and group IV (913 +/- 46 mg/dl). No significant differences in HDL cholesterol, LDL cholesterol, VLDL cholesterol, or triglyceride levels developed between the placebo and marine oil groups. Two-hour, hepatic total lipid, neutral steroid, fatty acid, bile acid, and cholesterol synthesis rates were not significantly affected by marine oil treatment. Thoracic aortic cholesterol content increased during cholesterol feeding (5.7 +/- 0.9 mg/gm vs. 1.1 +/- 0.05 mg/gm, group II vs. group I, p less than 0.05). Marine oil supplementation had no effect on the progressive accumulation of cholesterol in the thoracic aorta (28.8 +/- 2.5 mg/gm vs. 29.4 +/- 1.8 mg/gm, group IV vs. group III, p = 0.84). The abdominal aortic cholesterol contents were also similar. These results do not support the use of dietary marine oil supplements for the amelioration of lipid metabolism or the prevention of atherosclerosis.
膳食海洋油补充剂可能对动脉粥样硬化具有预防作用，尽管其对血脂、体内胆固醇代谢以及主动脉胆固醇蓄积的影响仍不明确。研究人员对33只新西兰白兔评估了每日服用海洋油（每千克提供100毫克二十碳五烯酸、59毫克二十二碳六烯酸和221毫克ω-3脂肪酸）的效果。六只动物（第一组）被立即处死。其余动物通过富含0.25%胆固醇的饮食诱导出稳定的高胆固醇血症。在此饮食7周后，六只动物被处死（第二组）。总血浆胆固醇显著升高（982±119毫克/分升对比55.6±7.1毫克/分升，均值±标准误，p<0.001）。其余动物随机每日接受自来水安慰剂（第三组，n = 12）或海洋油（第四组，n = 9）。3个月后，第二组（982±119毫克/分升）、第三组（965±54毫克/分升）和第四组（913±46毫克/分升）的总血浆胆固醇相似（p = 无显著差异）。安慰剂组和海洋油组之间的高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇或甘油三酯水平无显著差异。海洋油处理对两小时肝脏总脂质、中性类固醇、脂肪酸、胆汁酸和胆固醇合成率无显著影响。喂食胆固醇期间胸主动脉胆固醇含量增加（第二组对比第一组，5.7±0.9毫克/克对比1.1±0.05毫克/克，p<0.05）。补充海洋油对胸主动脉中胆固醇的逐渐蓄积无影响（第四组对比第三组，28.8±2.5毫克/克对比29.4±1.8毫克/克，p = 0.84）。腹主动脉胆固醇含量也相似。这些结果不支持使用膳食海洋油补充剂来改善脂质代谢或预防动脉粥样硬化。
