Vidyalakshmi S, Nirmal R Madhavan, Veeravarmal V, Santhadevy A, Aravindhan R
Senior Lecturer, Department of Oral and Maxillofacial Pathology, Indira Gandhi Institute of Dental Sciences , Pillaiyarkuppam, Pondicherry, India .
Professor, Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai University , Chidambaram, India .
J Clin Diagn Res. 2016 Jul;10(7):ZC05-7. doi: 10.7860/JCDR/2016/17074.8072. Epub 2016 Jul 1.
The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase.
To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group.
Buccal smears from 22 OLP and 10 control samples were stained in modified Feulgen-Rossenback reaction for micronuclei assay. Cytological evaluation of number of MicroNucleated cells (CMN), Total Number of Micronuclei (TMN) in micronucleated cells was done in both groups.
Frequency of micronucleated cells (CMN) when compared among the study and control group, a mean value of 4.27 ± 1.80 and 0.90 ± 0.88 were obtained respectively. On comparing the total number of micronuclei in the micronucleated cells (TMN) between the study and control groups, a mean value of 5.38 ± 2.42 and 1.5 ± 0.88 were obtained respectively.
There was a significant increase in the frequency of micronuclei and the micronucleated cells in the oral lichen planus as compared to normal individuals.
口腔扁平苔藓（OLP）的恶性转化率在0%至5.8%之间。扁平苔藓的口腔病变临床上多灶性出现，类似于高危恶性肿瘤的场癌化过程。颊黏膜微核细胞分析法（BMN分析法）提供了一个平台，通过在最早的微侵袭阶段评估核损伤标志物来识别高危个体。
评估口腔扁平苔藓病变个体颊黏膜脱落细胞中的DNA损伤，从而确定高危组。
对22份OLP样本和10份对照样本的颊黏膜涂片进行改良Feulgen-Rossenback反应染色，用于微核检测。对两组微核细胞数量（CMN）、微核细胞中的微核总数（TMN）进行细胞学评估。
研究组和对照组相比，微核细胞（CMN）的频率分别为4.27±1.80和0.90±0.88。研究组和对照组微核细胞中的微核总数（TMN）相比，平均值分别为5.38±2.42和1.5±0.88。
与正常个体相比，口腔扁平苔藓患者的微核和微核细胞频率显著增加。
