COPI囊泡和小管形成的重组。
Reconstitution of COPI Vesicle and Tubule Formation.
作者信息
Park Seung-Yeol, Yang Jia-Shu, Hsu Victor W
机构信息
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA, 02115, USA.
Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA.
出版信息
Methods Mol Biol. 2016;1496:63-74. doi: 10.1007/978-1-4939-6463-5_6.
The Golgi complex plays a central role in the intracellular sorting of proteins. Transport through the Golgi in the anterograde direction has been explained by cisternal maturation, while transport in the retrograde direction is attributed to vesicles formed by the coat protein I (COPI) complex. A more detailed understanding of how COPI acts in Golgi transport is being achieved in recent years, due in large part to a COPI reconstitution system. Through this approach, the mechanistic complexities of COPI vesicle formation are being elucidated. This approach has also uncovered a new mode of anterograde transport through the Golgi, which involves COPI tubules connecting the Golgi cisternae. We describe in this chapter the reconstitution of COPI vesicle and tubule formation from Golgi membrane.
高尔基体复合体在细胞内蛋白质分选过程中起着核心作用。顺向通过高尔基体的运输是通过潴泡成熟来解释的，而逆向运输则归因于由衣被蛋白I（COPI）复合体形成的囊泡。近年来，由于COPI重组系统，人们对COPI在高尔基体运输中的作用有了更详细的了解。通过这种方法，COPI囊泡形成的机制复杂性正在被阐明。这种方法还揭示了一种通过高尔基体的新的顺向运输模式，即通过连接高尔基体潴泡的COPI小管进行运输。在本章中，我们描述了从高尔基体膜重建COPI囊泡和小管形成的过程。
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