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COP I对于高尔基体潴泡成熟和动态变化至关重要。

COPI is essential for Golgi cisternal maturation and dynamics.

作者信息

Ishii Midori, Suda Yasuyuki, Kurokawa Kazuo, Nakano Akihiko

机构信息

Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Laboratory of Molecular Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

出版信息

J Cell Sci. 2016 Sep 1;129(17):3251-61. doi: 10.1242/jcs.193367. Epub 2016 Jul 21.

DOI:10.1242/jcs.193367
PMID:27445311
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5047698/
Abstract

Proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi and then sorted to their destinations. For their passage through the Golgi, one widely accepted mechanism is cisternal maturation. Cisternal maturation is fulfilled by the retrograde transport of Golgi-resident proteins from later to earlier cisternae, and candidate carriers for this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation of the transmembrane Golgi-resident proteins in living yeast cells. COPI temperature-sensitive mutants and induced degradation of COPI proteins were used to knockdown COPI function. For both methods, inactivation of COPI subunits Ret1 and Sec21 markedly impaired the transition from cis to medial and to trans cisternae. Furthermore, the movement of cisternae within the cytoplasm was severely restricted when COPI subunits were depleted. Our results demonstrate the essential roles of COPI proteins in retrograde trafficking of the Golgi-resident proteins and dynamics of the Golgi cisternae.

摘要

在内质网（ER）中合成的蛋白质被转运到高尔基体，然后被分类到它们的目的地。对于它们通过高尔基体的过程，一种广泛接受的机制是潴泡成熟。潴泡成熟是通过高尔基体驻留蛋白从晚期潴泡到早期潴泡的逆行转运来实现的，这种逆行转运的候选载体是衣被蛋白复合物I（COPI）包被的囊泡。我们通过对活酵母细胞中跨膜高尔基体驻留蛋白进行4D观察，直接研究了COPI在潴泡成熟中的功能。使用COPI温度敏感突变体和诱导COPI蛋白降解来敲低COPI功能。对于这两种方法，COPI亚基Ret1和Sec21的失活均显著损害了从顺面潴泡到中间潴泡再到反面潴泡的转变。此外，当COPI亚基被耗尽时，潴泡在细胞质内的移动受到严重限制。我们的结果证明了COPI蛋白在高尔基体驻留蛋白的逆行运输和高尔基体潴泡动态变化中的重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/2724d2644128/joces-129-193367-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/186677dda5ae/joces-129-193367-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/e24e532ab2fc/joces-129-193367-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/8e4eebf2a92b/joces-129-193367-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/c824f9c0f02a/joces-129-193367-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/87b30710d35f/joces-129-193367-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/1a7837a32c52/joces-129-193367-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/2724d2644128/joces-129-193367-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/186677dda5ae/joces-129-193367-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/e24e532ab2fc/joces-129-193367-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/8e4eebf2a92b/joces-129-193367-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/c824f9c0f02a/joces-129-193367-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/87b30710d35f/joces-129-193367-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/1a7837a32c52/joces-129-193367-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d6f/5047698/2724d2644128/joces-129-193367-g7.jpg

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本文引用的文献

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2
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Science. 2014 Oct 31;346(6209):1256898. doi: 10.1126/science.1256898.
3
Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER.顺面高尔基体与内质网出口位点的接触实现了内质网货物的捕获和转运。
RudLOV是一种光学同步的货物运输方法，揭示了dynasore的意外作用。
EMBO Rep. 2025 Feb;26(3):613-634. doi: 10.1038/s44319-024-00342-z. Epub 2024 Dec 10.
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Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast.在出芽酵母中高尔基体和内质网-高尔基体中间腔的时空剖析。
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