Chai Yi Wen, Lee Eu Han, Gubbe John D, Brekke John H
BRTI Life Sciences, Two Harbors, MN, United States of America.
PLoS One. 2016 Sep 15;11(9):e0162853. doi: 10.1371/journal.pone.0162853. eCollection 2016.
The development and utilization of three-dimensional cell culture platforms has been gaining more traction. Three-dimensional culture platforms are capable of mimicking in vivo microenvironments, which provide greater physiological relevance in comparison to conventional two-dimensional cultures. The majority of three-dimensional culture platforms are challenged by the lack of cell attachment, long polymerization times, and inclusion of undefined xenobiotics, and cytotoxic cross-linkers. In this study, we review the use of a highly defined material composed of naturally occurring compounds, hyaluronic acid and chitosan, known as Cell-Mate3DTM. Moreover, we provide an original measurement of Young's modulus using a uniaxial unconfined compression method to elucidate the difference in microenvironment rigidity for acellular and cellular conditions. When hydrated into a tissue-like hybrid hydrocolloid/hydrogel, Cell-Mate3DTM is a highly versatile three-dimensional culture platform that enables downstream applications such as flow cytometry, immunostaining, histological staining, and functional studies to be applied with relative ease.
三维细胞培养平台的开发与利用越来越受到关注。三维培养平台能够模拟体内微环境，与传统的二维培养相比，具有更高的生理相关性。大多数三维培养平台面临着细胞附着不足、聚合时间长、含有未定义的异生物质以及细胞毒性交联剂等挑战。在本研究中，我们回顾了一种由天然化合物透明质酸和壳聚糖组成的高度明确的材料——Cell-Mate3DTM的应用。此外，我们使用单轴无侧限压缩方法对杨氏模量进行了原始测量，以阐明无细胞和细胞条件下微环境刚度的差异。当水合形成类似组织的混合水胶体/水凝胶时，Cell-Mate3DTM是一个高度通用的三维培养平台，能够相对轻松地应用下游应用，如流式细胞术、免疫染色、组织学染色和功能研究。
