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在大鼠模型中，坐骨神经损伤后免疫细胞分布和免疫球蛋白水平会发生变化。

Immune cell distribution and immunoglobulin levels change following sciatic nerve injury in a rat model.

作者信息

Yuan Wei, Feng Xinhong

机构信息

Department of Spine Surgery, Aviation General Hospital of China Medical University, Beijing Institute of Translational Medicine, Chinese Academy of Sciences, No. 3 Anwai beiyuan Road, Chaoyang District, Beijing, 100012, China.

Department of Neurology, Beijing Tsinghua Changgung Hospital Medical Center, Tsinghua University, No. 168 Li Tang Rd. Dongxiaokou Town, Tiantongyuan Area, Changping District, Beijing, 102218, China.

出版信息

Iran J Basic Med Sci. 2016 Jul;19(7):794-9.

PMID:27635205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5010853/
Abstract

OBJECTIVES

To investigate the systemic and local immune status of two surgical rat models of sciatic nerve injury, a crushed sciatic nerve, and a sciatic nerve transection.

MATERIALS AND METHODS

Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups: sham-operation (control group), sciatic nerve crush, and sciatic nerve transaction. Sciatic nerve surgery was performed. The percentage of CD4(+) cells and the CD4(+)/CD8+ratio were determined by flow cytometry. Serum IgM and IgG levels were analyzed by ELISA. T-cells (CD3) and macrophages (CD68) in sciatic nerve tissue sections were identified through immunohistochemistry.

RESULTS

Compared to sham-operated controls, in rats that underwent nerve injury, the percentage of CD4(+) cells and the CD4(+)/CD8(+) ratio in the peripheral blood were significantly decreased 7 days after surgery, serum IgM levels were increased 14 days after surgery, and serum IgG levels were increased 21 days after surgery. There were a large number of CD3(+) cells and a small number of CD68(+) cells in sciatic nerve tissue sections 21 days after surgery, indicating T-cell and macrophage activation and infiltration. Local IgG deposition was also detected at the nerve injury site 21 days after surgery.

CONCLUSION

Rat humoral and cellular immune status changed following sciatic nerve injury, particularly with regard to the cellular immune response at the nerve injury site.

摘要

目的

研究坐骨神经损伤的两种手术大鼠模型（坐骨神经挤压伤和坐骨神经横断伤）的全身和局部免疫状态。

材料与方法

将24只成年雄性Sprague-Dawley大鼠随机分为三组：假手术组（对照组）、坐骨神经挤压伤组和坐骨神经横断伤组。进行坐骨神经手术。通过流式细胞术测定CD4(+)细胞百分比和CD4(+)/CD8+比值。采用酶联免疫吸附测定法分析血清IgM和IgG水平。通过免疫组织化学鉴定坐骨神经组织切片中的T细胞（CD3）和巨噬细胞（CD68）。

结果

与假手术对照组相比，神经损伤大鼠术后7天外周血中CD4(+)细胞百分比和CD4(+)/CD8(+)比值显著降低，术后14天血清IgM水平升高，术后21天血清IgG水平升高。术后21天坐骨神经组织切片中有大量CD3(+)细胞和少量CD68(+)细胞，表明T细胞和巨噬细胞活化并浸润。术后21天在神经损伤部位也检测到局部IgG沉积。

结论

坐骨神经损伤后大鼠体液和细胞免疫状态发生改变，尤其是神经损伤部位的细胞免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/593c1bb7f07a/IJBMS-19-794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/5d574061abe5/IJBMS-19-794-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/dbd0b24636a5/IJBMS-19-794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/593c1bb7f07a/IJBMS-19-794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/5d574061abe5/IJBMS-19-794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/4b7406d3e1ab/IJBMS-19-794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/f68ed451dd61/IJBMS-19-794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/dbd0b24636a5/IJBMS-19-794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acab/5010853/593c1bb7f07a/IJBMS-19-794-g005.jpg

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