Guo Yuanheng, Cao Lili, Zhao Qingsheng, Zhang Lijun, Chen Jinjin, Liu Boyan, Zhao Bing
Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing,100049, PR China.
Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China.
Int J Biol Macromol. 2016 Dec;93(Pt A):678-685. doi: 10.1016/j.ijbiomac.2016.09.039. Epub 2016 Sep 13.
To research the preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharides from Cistanche deserticola (CDPs), three polysaccharide fractions, CDP-A, CDP-B and CDP-C, were obtained by successively membrane filtration (microfiltration, ultrafiltration and nanofiltration). Molecular weights, monosaccharide compositions, purities and IR spectra of the three fractions were analyzed. Results showed that CDP-C contained higher proportion of galacturonic acid (GalUA) than CDP-B and CDP-A. Antioxidant activities were also analyzed and the results revealed that CDP-C possessed the highest activity. Thus, hepatoprotective activity of CDP-C was studied further. In vitro research, CDP-C promoted viability of HepG2 cells. In vivo research, CDP-C ameliorated the alterations induced by alcohol, including serological indexes (alanine transaminase, acid phosphatase, γ-glutamyl transpeptidase and triglyceride) and hepatic indicators (superoxide dismutase, malondialdehyde, glutathione S-transferase and triglyceride) in model animals. The prominent microvesicular steatosis and mild necrosis in hepatic histopathology of model animals were also attenuated by CDP-C administration. These findings indicated that CDP-C possessed hepatoprotective activity against chronic hepatic injury induced by alcohol. The underlying mechanism might be that CDP-C can reduce the contents of MDA and TG, and modulate the activities of the relative enzyme. This property might associate with GalUA in CDP-C.
为研究肉苁蓉多糖（CDPs）的初步特性、抗氧化及保肝活性，通过依次进行膜过滤（微滤、超滤和纳滤）获得了三个多糖级分，即CDP-A、CDP-B和CDP-C。分析了这三个级分的分子量、单糖组成、纯度及红外光谱。结果表明，CDP-C中半乳糖醛酸（GalUA）的比例高于CDP-B和CDP-A。还分析了抗氧化活性，结果显示CDP-C具有最高活性。因此，进一步研究了CDP-C的保肝活性。体外研究中，CDP-C促进了HepG2细胞的活力。体内研究中，CDP-C改善了酒精诱导的改变，包括模型动物的血清学指标（谷丙转氨酶、酸性磷酸酶、γ-谷氨酰转肽酶和甘油三酯）和肝脏指标（超氧化物歧化酶、丙二醛、谷胱甘肽S-转移酶和甘油三酯）。模型动物肝脏组织病理学中明显的微泡性脂肪变性和轻度坏死也因给予CDP-C而减轻。这些发现表明，CDP-C对酒精诱导的慢性肝损伤具有保肝活性。潜在机制可能是CDP-C可降低丙二醛和甘油三酯的含量，并调节相关酶的活性。这一特性可能与CDP-C中的GalUA有关。
