Zhou Xin, Deng Qingfang, Chen Huaguo, Hu Enming, Zhao Chao, Gong Xiaojian
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China.
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, 116 Baoshan North Rd., Guiyang, Guizhou, 550001, PR China.
Int J Biol Macromol. 2017 Sep;102:60-67. doi: 10.1016/j.ijbiomac.2017.03.083. Epub 2017 Mar 18.
Crude polysaccharides of Mori Fructus (MFPs) were found to have anti-inflammatory antioxidant, and immuno-enhancing activities. However, the structure of the polysaccharides was ambiguous and its holistic hepatic protection evaluation was defective. This study was conducted to illustrate the characterization of MFPs, and evaluate its hepatoprotective activities. The results found that MFPs contained 67.93±1.18% carbohydrates, 31.03±0.54% uronic acid, and little protein and sulfate. The average molecular weight was ranging from 112.2kDa to 181.9kDa. Monosaccharide component analysis indicated that MFPs was mainly composed of glucose, galacturonic acid, rhamnose and galactose. Both the acute and subacute alcoholic-induced liver injury animal models were adopted to evaluate the MFPs's hepatoprotective activity. After administration of MFPs, both serological indexes (aspartate aminotransferase and alanine aminotransferase) and hepatic indicators (glutathione, superoxide dismutase, glutathione peroxidase and malondialdehyde) were improved by comparing with the non-MFPs group. The hepatic histopathology results also showed a prominent lipid degeneration and microvesicular steatosis attenuation in the MFPs groups. These outstanding hepatic protecting activities of MFPs might be related to its activation of ethanol dehydrogenase, elimination of free radicals and/or inhibition of lipid peroxidation capacities. MFPs could be important active substances for preventing and remedying liver injury.
桑椹粗多糖（MFPs）具有抗炎、抗氧化和免疫增强活性。然而，其多糖结构尚不明确，整体肝脏保护评价也存在缺陷。本研究旨在阐明MFPs的特性，并评估其肝脏保护活性。结果发现，MFPs含有67.93±1.18%的碳水化合物、31.03±0.54%的糖醛酸，蛋白质和硫酸盐含量很少。平均分子量在112.2kDa至181.9kDa之间。单糖成分分析表明，MFPs主要由葡萄糖、半乳糖醛酸、鼠李糖和半乳糖组成。采用急性和亚急性酒精性肝损伤动物模型评估MFPs的肝脏保护活性。给予MFPs后，与未给予MFPs组相比，血清学指标（天冬氨酸转氨酶和丙氨酸转氨酶）和肝脏指标（谷胱甘肽、超氧化物歧化酶、谷胱甘肽过氧化物酶和丙二醛）均有所改善。肝脏组织病理学结果也显示，MFPs组脂质变性和微泡性脂肪变明显减轻。MFPs这些显著的肝脏保护活性可能与其激活乙醇脱氢酶、清除自由基和/或抑制脂质过氧化能力有关。MFPs可能是预防和治疗肝损伤的重要活性物质。
