Santos Silvana Ornelas, Fontes Jonathan L M, Laranjeira Daniela F, Vassallo José, Barrouin-Melo Stella Maria, Dos-Santos Washington L C
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz, Salvador, BA, Brazil;, Brazil; Laboratório de Infectologia Veterinária, Hospital de Medicina Veterinária, Universidade Federal da Bahia (UFBA), Salvador, BA, Brazil.
Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz, Salvador, BA, Brazil;, Brazil.
Comp Immunol Microbiol Infect Dis. 2016 Oct;48:87-92. doi: 10.1016/j.cimid.2016.08.007. Epub 2016 Aug 30.
Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher's test, P<0.02) and a higher density of plasma cells (score 3, Fisher's test, P<0.02) than uninfected dogs (5/7 exhibiting lymphoid follicles and a plasma cell score of 1). The dogs with Leishmania infection also presented with granulomas (8/13) and infected macrophages (5/13). These differences in the histological presentations of spleen tissue from infected and uninfected dogs corresponded to changes observed in conventional histology. Hence, the cell-block technique described here may be used in the follow-up care and study of dogs with ZVL and other diseases in both clinical practice and research.
严重形式的人畜共患内脏利什曼病（ZVL）与脾脏结构破坏有关。然而，脾脏组织学研究需要进行脾切除术或尸检。在这项工作中，我们提出了一种微创细胞块技术，用于研究患有ZVL的犬类的脾脏组织学。我们检查了13只感染婴儿利什曼原虫的犬和7只未感染的犬。与未感染的犬（5/7有淋巴滤泡且浆细胞评分为1）相比，感染利什曼原虫的犬淋巴滤泡出现频率较低（2/13，Fisher检验，P<0.02），浆细胞密度较高（评分为3，Fisher检验，P<0.02）。感染利什曼原虫的犬还出现肉芽肿（8/13）和被感染的巨噬细胞（5/13）。感染和未感染犬的脾脏组织学表现差异与传统组织学观察到的变化一致。因此，本文所述的细胞块技术可用于ZVL及其他疾病犬的临床实践和研究中的后续护理及研究。
