Dalmasso Cécile, Oger Philippe, Selva Gwendoline, Courtine Damien, L'Haridon Stéphane, Garlaschelli Alexandre, Roussel Erwan, Miyazaki Junichi, Reveillaud Julie, Jebbar Mohamed, Takai Ken, Maignien Lois, Alain Karine
Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM)-UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Place Nicolas Copernic, F-29280 Plouzané, France; CNRS, IUEM-UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Place Nicolas Copernic, F-29280 Plouzané, France; Ifremer, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Technopôle Pointe du diable, F-29280 Plouzané, France.
Université de Lyon, INSA Lyon, CNRS UMR 5240, 11 Avenue Jean Capelle, F-69621 Villeurbanne, France.
Syst Appl Microbiol. 2016 Oct;39(7):440-444. doi: 10.1016/j.syapm.2016.08.003. Epub 2016 Aug 26.
A novel strictly anaerobic, hyperthermophilic archaeon, designated strain CDGS, was isolated from a deep-sea hydrothermal vent in the Cayman Trough at 4964m water depth. The novel isolate is obligate anaerobe and grows chemoorganoheterotrophically with stimulation of growth by sulphur containing compounds. Its growth is optimal at 75°C, pH 6.0 and under a pressure of 50MPa. It possesses the broadest hydrostatic pressure range for growth that has ever been described for a microorganism. Its genomic DNA G+C content is 51.11mol%. The novel isolate belongs to the genus Thermococcus. Phylogenetic analyses indicated that it is most closely related to Thermococcus barossii DSM17882 based on its 16S rRNA gene sequence, and to 'Thermococcus onnurineus' NA1 based on its whole genome sequence. The average nucleotide identity scores with these strains are 77.66% for T. barossii and 84.84% for 'T. onnurineus', respectively. Based on the draft whole genome sequence and phenotypic characteristics, strain CDGS is suggested to be separated into a novel species within the genus Thermococcus, with proposed name Thermococcus piezophilus (type strain CDGS=ATCC TSD-33=UBOCC 3296).
从开曼海沟水深4964米处的深海热液喷口中分离出一种新型严格厌氧、嗜热古菌，命名为CDGS菌株。该新型分离株为专性厌氧菌，以含硫化合物刺激生长，进行化能有机异养生长。其最适生长温度为75°C，pH值为6.0，压力为50MPa。它具有微生物中描述过的最宽的生长静水压力范围。其基因组DNA的G+C含量为51.11mol%。该新型分离株属于嗜热栖热菌属。系统发育分析表明，基于其16S rRNA基因序列，它与巴罗斯嗜热栖热菌DSM17882关系最为密切；基于其全基因组序列，它与“温泉嗜热栖热菌”NA1关系最为密切。与这些菌株的平均核苷酸同一性得分分别为：与巴罗斯嗜热栖热菌为77.66%，与“温泉嗜热栖热菌”为84.84%。基于全基因组草图序列和表型特征，建议将CDGS菌株分离为嗜热栖热菌属内的一个新物种，命名为嗜压嗜热栖热菌（模式菌株CDGS=ATCC TSD-33=UBOCC 3296）。
