Jørgensen J C, Sheikh S P, Forman A, Nørgård M, Schwartz T W, Ottesen B
Department of Obstetrics and Gynecology, Rigshospitalet, University of Copenhagen, Denmark.
Am J Physiol. 1989 Aug;257(2 Pt 1):E220-7. doi: 10.1152/ajpendo.1989.257.2.E220.
The distribution, localization, and smooth muscle effects of neuropeptide Y (NPY) were studied in the human female genital tract. High concentrations of NPY immunoreactivity were demonstrated in the uterine artery, the ovary, the fallopian tube, cervix, and the vagina. The NPY immunoreactivity was confined to nerve fibers. The highest density of nerve fibers was observed in relation to blood vessels, although some NPY-immunoreactive nerves were also seen close to nonvascular smooth muscle. The NPY-immunoreactive material throughout the genital tract was identical to synthetic amidated human NPY with regard to size, hydrophobicity, and charge as evaluated by gel filtration, high-performance liquid chromatography, and isoelectric focusing. NPY (10(-10) to 10(-6) M) exerted a direct vasoconstrictory effect on small arteries dissected from the cervix and an additive effect of NPY and norepinephrine responses was observed. Exogenous NPY did not have a direct effect on nonvascular smooth muscle specimens from the fallopian tube or the myometrium. The close relation between NPY-immunoreactive nerves and blood vessels, the presence of NPY-immunoreactive material identical to amidated synthetic human NPY, and the vasoconstrictory effects of NPY indicate that NPY is involved in the regulation of the blood flow in the human female genital tract.
研究了神经肽Y（NPY）在女性生殖道中的分布、定位及对平滑肌的作用。在子宫动脉、卵巢、输卵管、宫颈和阴道中均检测到高浓度的NPY免疫反应性。NPY免疫反应性局限于神经纤维。尽管在非血管平滑肌附近也可见到一些NPY免疫反应性神经，但神经纤维密度最高处与血管相关。通过凝胶过滤、高效液相色谱和等电聚焦评估，整个生殖道中的NPY免疫反应性物质在大小、疏水性和电荷方面与合成的酰胺化人NPY相同。NPY（10⁻¹⁰至10⁻⁶M）对从宫颈分离的小动脉有直接血管收缩作用，并且观察到NPY和去甲肾上腺素反应具有相加效应。外源性NPY对来自输卵管或子宫肌层的非血管平滑肌标本没有直接作用。NPY免疫反应性神经与血管之间的密切关系、与酰胺化合成人NPY相同的NPY免疫反应性物质的存在以及NPY的血管收缩作用表明，NPY参与女性生殖道血流的调节。
