Tenmoku S, Ottesen B, O'Hare M M, Sheikh S, Bardrum B, Hansen B, Walker B, Murphy R F, Schwartz T W
Department of Clinical Chemistry, Copenhagen, Denmark.
Peptides. 1988 Mar-Apr;9(2):269-75. doi: 10.1016/0196-9781(88)90259-8.
The occurrence, molecular characteristics and biological function of neuropeptide Y (NPY) has been studied in the female genital tract of non-pregnant rabbits. NPY immunoreactivity was demonstrated throughout the genital tract. Maximum concentrations were found in the salpinx (fallopian tube), 570 pmol/g (median) lower within the uterine body (1.5 pmol/g), cervix (2.8 pmol/g) and vagina (3.6 pmol/g). In vitro, NPY had a dose-dependent stimulatory effect on non-vascular smooth muscle (ED50 10(-9) mol/l) as studied by myometrial tension recordings. In vivo, NPY (50 pmol/min.kg) induced a dose-related, non-adrenergic and non-cholinergic decrease in myometrial blood flow. Small C-terminal (NPY31-36) or N-terminal (NPY1-16) fragments of NPY had no effect on myometrial blood flow. NPY was found to interact with the smooth muscle effect of VIP; the presence of VIP (10(-8) mol/l) counteracted the contraction elicited by NPY (10(-8) mol/l) returning the response to control value. VIP and NPY displayed a similar physiological antagonism on myometrial blood flow. There was a clear difference in the response to VIP and NPY as the effect of NPY on myometrial blood flow first appeared after a lag period of 2 minutes whereas the effect of VIP was almost instantaneous. It is concluded that NPY and VIP may interact in the local nervous control of genital functions.
已在未孕兔的雌性生殖道中研究了神经肽Y（NPY）的发生、分子特征及生物学功能。在整个生殖道中均证实有NPY免疫反应性。输卵管中的浓度最高（中位数为570 pmol/g），子宫体（1.5 pmol/g）、子宫颈（2.8 pmol/g）和阴道（3.6 pmol/g）中的浓度较低。通过子宫肌层张力记录研究发现，体外实验中，NPY对非血管平滑肌有剂量依赖性刺激作用（半数有效剂量为10⁻⁹ mol/l）。体内实验中，NPY（50 pmol/min·kg）可引起子宫肌层血流呈剂量相关的、非肾上腺素能和非胆碱能性减少。NPY的小C末端片段（NPY31 - 36）或N末端片段（NPY1 - 16）对子宫肌层血流无影响。发现NPY与血管活性肠肽（VIP）对平滑肌的作用相互影响；VIP（10⁻⁸ mol/l）的存在可抵消NPY（10⁻⁸ mol/l）引起的收缩，使反应恢复到对照值。VIP和NPY在子宫肌层血流方面表现出类似的生理拮抗作用。对VIP和NPY的反应存在明显差异，因为NPY对子宫肌层血流的作用在2分钟的延迟期后才首次出现，而VIP的作用几乎是即时的。结论是，NPY和VIP可能在生殖功能的局部神经控制中相互作用。
