Maier Tanja Verena, Schmitt-Kopplin Philippe
Department of Environmental Sciences, Helmholtz Zentrum München, Neuherberg, 85764, Germany.
Department of Environmental Sciences, Helmholtz Zentrum Munchen, Neuherberg, Germany.
Methods Mol Biol. 2016;1483:437-70. doi: 10.1007/978-1-4939-6403-1_21.
Metabolomics is an analytical toolbox to describe (all) low-molecular-weight compounds in a biological system, as cells, tissues, urine, and feces, as well as in serum and plasma. To analyze such complex biological samples, high requirements on the analytical technique are needed due to the high variation in compound physico-chemistry (cholesterol derivatives, amino acids, fatty acids as SCFA, MCFA, or LCFA, or pathway-related metabolites belonging to each individual organism) and concentration dynamic range. All main separation techniques (LC-MS, GC-MS) are applied in routine to metabolomics hyphenated or not to mass spectrometry, and capillary electrophoresis is a powerful high-resolving technique but still underused in this field of complex samples. Metabolomics can be performed in the non-targeted way to gain an overview on metabolite profiles in biological samples. Targeted metabolomics is applied to analyze quantitatively pre-selected metabolites. This chapter reviews the use of capillary electrophoresis in the field of metabolomics and exemplifies solutions in metabolite profiling and analysis in urine and plasma.
代谢组学是一种分析工具箱，用于描述生物系统（如细胞、组织、尿液、粪便以及血清和血浆）中的（所有）低分子量化合物。由于化合物的物理化学性质（胆固醇衍生物、氨基酸、作为短链脂肪酸、中链脂肪酸或长链脂肪酸的脂肪酸，或属于每个生物体的途径相关代谢物）和浓度动态范围存在很大差异，因此分析此类复杂的生物样品对分析技术有很高的要求。所有主要的分离技术（液相色谱-质谱联用、气相色谱-质谱联用）在代谢组学中常规应用，无论是否与质谱联用，而毛细管电泳是一种强大的高分辨率技术，但在这个复杂样品领域仍未得到充分利用。代谢组学可以以非靶向方式进行，以全面了解生物样品中的代谢物谱。靶向代谢组学用于定量分析预先选择的代谢物。本章回顾了毛细管电泳在代谢组学领域的应用，并举例说明了尿液和血浆中代谢物谱分析和分析的解决方案。
