多花 Allanblackia：一种具有血管舒张特性的壮阳植物。

Allanblackia floribunda Oliv.: An aphrodisiac plant with vasorelaxant properties.

作者信息

Brusotti G, Papetti A, Serra M, Temporini C, Marini E, Orlandini S, Sanda A Kada, Watcho P, Kamtchouing P

机构信息

Department of Drug Sciences, University of Pavia, Pavia, Italy; Center for Studies and Researches in Ethnobiopharmacy and Traditional and Complementary Medicines University of Pavia (CEMEC), Pavia, Italy.

出版信息

J Ethnopharmacol. 2016 Nov 4;192:480-485. doi: 10.1016/j.jep.2016.09.033. Epub 2016 Sep 17.

DOI:10.1016/j.jep.2016.09.033

PMID:27647010

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Allanblackia floribunda Oliv. is one of the most commonly used medicinal plant in Cameroon. The stem bark of the plant is traditionally used for its aphrodisiac and antihypertensive properties.

AIM OF THE STUDY

To validate the traditional uses of Allanblackia floribunda stem bark ethanol extract through the evaluation of their aphrodisiac and vasorelaxant properties.

MATERIALS AND METHODS

The extract's ability to increase sexual desire and the frequencies of erection (mount), intromission and prolonged latency of ejaculation were studied on adult male rats. The vasodilator effect was investigated using isolated rat aorta rings. Tests were conducted using fractions obtained by reverse phase column-chromatography (CC), after the acquisition of the HPLC fingerprint of the ethanol extract, resulted the most active in previous studies.

RESULTS

The CC allowed the isolation of five fractions whose aphrodisiac and vasodilator activities were tested and compared with those of the whole extract. Four compounds were identified and characterized, three of them, Fukugiside, Morelloflavone and Volkensiflavone, are secondary metabolites known to be in Allanblackia floribunda; the fourth, Spicataside, is a biflavonoid glycoside known to be present in the genus Garcinia but never found neither in Allanblackia floribunda nor in Allanblackia genus. The crude ethanolic extract (CEE) induced a relaxation on aorta rings with EC=11±2μg/mL and Morelloflavone displayed a similar activity with EC=42±6μg/mL; for all the other compounds only the vasodilation % at the maximum concentration assessable (90μg/mL) was determined: 30±8 (Fukugiside), 24±6 (Spicataside), 33±4 (Morelloflavone+Volkensiflavone), 47±1 (Volkensiflavone). Regarding the activity on male sexual behaviour, only CEE and Fukugiside showed activity in the 9 parameters evaluated.

CONCLUSIONS

These results may support the traditional uses of Allanblackia floribunda as aphrodisiac plant with antihypertensive properties suggesting the phytocomplex as responsible for the claimed activity.

摘要

民族药理学相关性

多花 Allanblackia floribunda Oliv. 是喀麦隆最常用的药用植物之一。该植物的茎皮传统上因其壮阳和降血压特性而被使用。

研究目的

通过评估多花 Allanblackia floribunda 茎皮乙醇提取物的壮阳和血管舒张特性，验证其传统用途。

材料与方法

在成年雄性大鼠上研究该提取物增加性欲以及勃起（爬跨）频率、插入频率和射精延迟时间的能力。使用离体大鼠主动脉环研究血管舒张作用。在获得乙醇提取物的高效液相色谱指纹图谱后，使用反相柱色谱（CC）得到的馏分进行测试，这些馏分在先前研究中显示出最活跃的效果。

结果

通过柱色谱分离得到五个馏分，测试并比较了它们的壮阳和血管舒张活性与整个提取物的活性。鉴定并表征了四种化合物，其中三种，福木苷、杨梅黄酮和伏康西黄酮，是已知存在于多花 Allanblackia floribunda 中的次生代谢产物；第四种，穗花杉苷，是一种双黄酮糖苷，已知存在于藤黄属植物中，但从未在多花 Allanblackia floribunda 或 Allanblackia 属中发现。粗乙醇提取物（CEE）对主动脉环有舒张作用，EC = 11±2μg/mL，杨梅黄酮表现出类似活性，EC = 42±6μg/mL；对于所有其他化合物，仅测定了可评估的最大浓度（90μg/mL）下的血管舒张百分比：30±8（福木苷）、24±6（穗花杉苷）、33±4（杨梅黄酮 + 伏康西黄酮）、47±1（伏康西黄酮）。关于对雄性性行为的活性，只有 CEE 和福木苷在评估的 9 个参数中显示出活性。