Azebaze Anatole Guy Blaise, Teinkela Jean Emmanuel Mbosso, Nguemfo Edwige Laure, Valentin Alexis, Dongmo Alain Bertrand, Vardamides Juliette Catherine
Department of Chemistry, Faculty of Science, University of Douala, P.O. Box. 24157, Douala, Cameroon.
Department of Chemistry, Faculty of Science, University of Douala, P.O. Box. 24157, Douala, Cameroon; Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box. 2701 Douala, Cameroon.
Afr Health Sci. 2015 Sep;15(3):835-40. doi: 10.4314/ahs.v15i3.18.
Plasmodium falciparum, one of the causative agents of malaria, has high adaptability through mutation and is resistant to many types of anti-malarial drugs. This study presents an in vitro assessment of the antiplasmodial activity of some phenolic compounds isolated from plants of the genus Allanblackia.
Tests were performed on well plates filled with a fixed parasitized erythrocytes volume. Compounds to be tested were then added in wells. After incubation, tritiated hypoxanthine is added and the plates were returned to the incubator. After thawing, the nucleic acids are collected. Inhibitory Concentration 50 (IC50) was determined by linear interpolation.
From Allanblackia floribunda, have been isolated and characterized 1,7-dihydroxyxanthone 1, macluraxanthone 4, morelloflavone 9, Volkensiflavone 10 and morelloflavone 7-O-glucoside 11; from Allanblackia monticola, α-mangosine 2, rubraxanthone 3, allaxanthone C 5, norcowanine 6, tovophiline A 7, allaxanthone B 8 and from Allanblackia gabonensis, 1,7-dihydroxyxanthone 1. Six of them were evaluated for their antimalarial properties. The most active compound, macluraxanthone, presented a very interesting activity, with an IC50 of 0.36 and 0.27 µg/mL with the F32 and FcM29 strains respectively.
This work confirms that species of Allanblackia genus are medicinally important plants containing many biologically active compounds that can be used effectively as antiplasmodial.
恶性疟原虫是疟疾的致病因子之一，通过突变具有高度适应性，并且对多种抗疟药物耐药。本研究对从阿兰藤黄属植物中分离出的一些酚类化合物的抗疟活性进行了体外评估。
在装有固定体积受感染红细胞的微孔板上进行试验。然后将待测试的化合物加入孔中。孵育后，加入氚标记的次黄嘌呤，再将微孔板放回培养箱。解冻后，收集核酸。通过线性插值法测定半数抑制浓度（IC50）。
从多花阿兰藤黄中分离并鉴定出1,7 - 二羟基呫吨酮1、桑橙黄素4、莫雷黄酮9、伏氏黄酮10和莫雷黄酮7 - O - 葡萄糖苷11；从山地阿兰藤黄中分离出α - 芒果苷2、红藤黄醌3、异藤黄醌C 5、降柯瓦宁6、托菲林A 7、异藤黄醌B 8；从加蓬阿兰藤黄中分离出1,7 - 二羟基呫吨酮1。对其中六种化合物的抗疟特性进行了评估。活性最强的化合物桑橙黄素表现出非常有趣的活性，对F32和FcM29菌株的IC50分别为0.36和0.27μg/mL。
本研究证实阿兰藤黄属植物是具有药用重要性的植物，含有许多具有生物活性的化合物，可有效用作抗疟药物。
