Lebret Karen, Schroeder Joanna, Balestreri Cecilia, Highfield Andrea, Cummings Denise, Smyth Tim, Schroeder Declan
Marine Biological Association, Citadel Hill, Plymouth, UK; Department of Ecology and Genetics/Limnology, Uppsala University, Uppsala, Sweden.
Marine Biological Association, Citadel Hill, Plymouth, UK.
Mar Genomics. 2016 Oct;29:39-43. doi: 10.1016/j.margen.2016.09.001. Epub 2016 Sep 16.
The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.
近年来，新一代测序技术的迅速发展使得研究微生物群落的方法日益多样化；然而，这些不同方法所产生的数据之间的可比性究竟如何，仍不明确。在本研究中，我们比较了通过两种不同的16s扩增子测序技术所确定的细菌群落的分类组成和季节动态变化：一种是使用454焦磷酸测序技术对16s rRNA基因的V6区域进行测序，另一种是使用Illumina Hiseq 2500平台对16s rRNA基因的V4区域进行测序。我们观察到在所有分类水平上相对丰度之间存在显著差异；然而，不同方法之间各门类的季节动态变化在很大程度上是一致的。本研究强调，在比较不同方法所产生的数据集时必须谨慎。
