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用ATB 32 Staph系统和传统试验对猪葡萄球菌进行鉴定。

Characterization of Staphylococcus hyicus with the ATB 32 Staph system and with conventional tests.

作者信息

Lämmler C

机构信息

Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.

出版信息

J Clin Microbiol. 1991 Jun;29(6):1221-4. doi: 10.1128/jcm.29.6.1221-1224.1991.

DOI:10.1128/jcm.29.6.1221-1224.1991
PMID:1864941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269973/
Abstract

The ATB 32 Staph system correctly identified 45 of 54 Staphylococcus hyicus cultures isolated from pigs and cattle. The biochemical profiles of the remaining nine cultures were not listed in the product data base. The 40 porcine and 14 bovine cultures resulted in three and seven different biochemical profiles, respectively. In parallel experiments, almost all S. hyicus cultures showed hemolytic reactions on chocolate agar, had CAMP-like reactivities in the zone of lysis of the staphylococcal beta-lysin, and had bacteriolytic properties on Micrococcus luteus cells. In addition, the S. hyicus cultures were unpigmented, were DNase positive, expressed an S. hyicus-specific teichoic acid, and were usually coagulase positive in porcine plasma. Most of the porcine strains were protein A positive, and two porcine cultures were clumping factor positive.

摘要

ATB 32葡萄球菌系统正确鉴定出了从猪和牛身上分离出的54株猪葡萄球菌培养物中的45株。其余9株培养物的生化谱未列在产品数据库中。40株猪源培养物和14株牛源培养物分别产生了3种和7种不同的生化谱。在平行实验中,几乎所有猪葡萄球菌培养物在巧克力琼脂上都表现出溶血反应,在葡萄球菌β-溶血素的裂解区具有类CAMP反应性,并且对藤黄微球菌细胞具有溶菌特性。此外,猪葡萄球菌培养物无色素,DNase阳性,表达猪葡萄球菌特异性磷壁酸,并且在猪血浆中通常凝固酶阳性。大多数猪源菌株蛋白A阳性,有两株猪源培养物凝聚因子阳性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0735/269973/b72ff31d53bf/jcm00042-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0735/269973/3a930fdb324c/jcm00042-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0735/269973/b72ff31d53bf/jcm00042-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0735/269973/3a930fdb324c/jcm00042-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0735/269973/b72ff31d53bf/jcm00042-0148-b.jpg

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本文引用的文献

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J Clin Microbiol. 1981 Dec;14(6):671-3. doi: 10.1128/jcm.14.6.671-673.1981.
2
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J Clin Microbiol. 1982 Jun;15(6):984-6. doi: 10.1128/jcm.15.6.984-986.1982.
3
Staphylococcus hyicus in skin lesions of horses.
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J Clin Microbiol. 1997 Dec;35(12):3116-21. doi: 10.1128/jcm.35.12.3116-3121.1997.
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Simple enzymatic method for rapid identification of a Staphylococcus aureus subspecies aureus biovar.快速鉴定金黄色葡萄球菌金黄色亚种生物变种的简易酶法
Eur J Clin Microbiol Infect Dis. 1997 Sep;16(9):689-92. doi: 10.1007/BF01708562.
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J Clin Microbiol. 1996 Dec;34(12):3075-84. doi: 10.1128/jcm.34.12.3075-3084.1996.
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