Jothiprakasam Vinoth, Sambantham Murugan, Chinnathambi Stalin, Vijayaboopathi Singaravel
CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
Arch Oral Biol. 2017 Jan;73:21-24. doi: 10.1016/j.archoralbio.2016.09.003. Epub 2016 Sep 14.
Biofilm of Candida tropicalis denote as a complex cellular congregation with major implication in pathogenesis. This lifestyle of fungus as a biofilm can inhibit immune system and antifungal therapy in treatment of infectious disease especially medical device associated chronic disease. In this study effects of Zinc Oxide (ZnO) nanoparticles and EDTA were evaluated on C. tropicalis biofilm by using different techniques. ZnO nanoparticles were synthesized from Egg albumin.
To assay the formation of biofilm of yeast cells like Fluconazole-susceptible C. tropicalis (ATCC 13,803) and fluconazole-resistant standard strains of C. tropicalis (ATCC 750) were grown in 24 well plates and antifungal effect of ZnO and EDTA were evaluated on C. tropicalis biofilm using ATP bioluminescence and tetrasodium salt (XTT) reduction assays.
Synthesized ZnO NPs and EDTA had effective antifungal properties at the concentration of 5.2, 8.6μg/ml for Fluconazole susceptible strain and 5.42, 10.8μg/ml Fluconazole resistant strains of C. tropicalis biofilms compared to fluconazole drug.
In present study we conclude, ZnO considered as a new agent in field of prevention C. tropicalis biofilms especially biofilms formed surface of medical device.
热带假丝酵母菌生物膜是一种复杂的细胞聚集体，在发病机制中具有重要意义。真菌的这种生物膜生活方式可抑制免疫系统和抗真菌治疗，尤其是在治疗与医疗器械相关的慢性疾病等传染病时。在本研究中，采用不同技术评估了氧化锌（ZnO）纳米颗粒和乙二胺四乙酸（EDTA）对热带假丝酵母菌生物膜的影响。ZnO纳米颗粒由蛋清蛋白合成。
为检测酵母细胞生物膜的形成，将氟康唑敏感的热带假丝酵母菌（ATCC 13803）和氟康唑耐药的热带假丝酵母菌标准菌株（ATCC 750）在24孔板中培养，并用ATP生物发光法和四钠盐（XTT）还原法评估ZnO和EDTA对热带假丝酵母菌生物膜的抗真菌作用。
与氟康唑药物相比，合成的ZnO纳米颗粒和EDTA对热带假丝酵母菌生物膜的氟康唑敏感菌株在浓度为5.2、8.6μg/ml时具有有效的抗真菌特性，对氟康唑耐药菌株在浓度为5.42、10.8μg/ml时具有有效的抗真菌特性。
在本研究中我们得出结论，ZnO被认为是预防热带假丝酵母菌生物膜，尤其是在医疗器械表面形成的生物膜领域的一种新型药物。
