Ottestad L, Tveit K M, Hannisdal E, Skrede M, Nesland J M, Gundersen S, Pihl A
Department of Biochemistry, Institute for Cancer Research, Oslo, Norway.
Br J Cancer. 1989 Aug;60(2):216-9. doi: 10.1038/bjc.1989.254.
To study whether colony growth in vitro reflects the prognosis of breast cancer patients, specimens from a total number of 138 patients with primary breast carcinomas were cultivated in the Courtenay-Mills soft agar method. The plating efficiency (PE) values were related to various clinical and histopathological parameters. No significant correlation was found between colony forming ability and menopausal status, histopathology, TNM-status or steroid hormone receptor status. The crude survival of the patients was not significantly correlated to the in vitro growth of the tumours; neither was there any difference in relapse-free survival between patients whose tumours failed to grow in vitro and those having growing tumours (PE greater than 0). A multivariate survival analysis of 115 patients with primary tumours without distant metastases revealed that the PE was not a significant independent prognostic indicator, as it gave no additional prognostic information above that of node and ER status. It is concluded that routine measurement of colony formation in vitro is not warranted in the management of breast cancer.
为研究体外集落生长是否反映乳腺癌患者的预后，采用考特尼 - 米尔斯软琼脂法培养了138例原发性乳腺癌患者的标本。接种效率（PE）值与各种临床和组织病理学参数相关。未发现集落形成能力与绝经状态、组织病理学、TNM分期或类固醇激素受体状态之间存在显著相关性。患者的总生存率与肿瘤的体外生长无显著相关性；肿瘤在体外未生长的患者与肿瘤生长的患者（PE大于0）之间的无复发生存率也无差异。对115例无远处转移的原发性肿瘤患者进行的多因素生存分析显示，PE不是一个显著的独立预后指标，因为它没有提供超出淋巴结和雌激素受体状态的额外预后信息。结论是，在乳腺癌的管理中，不建议常规测量体外集落形成。
