柠檬烯对希瓦氏菌属 H16 异养生长和聚羟基丁酸酯生产的影响。
Effect of limonene on the heterotrophic growth and polyhydroxybutyrate production by Cupriavidus necator H16.
机构信息
School of Chemical Engineering and Analytical Science, The Mill, The University of Manchester, Manchester M13 9PL, UK.
School of Chemical Engineering and Analytical Science, The Mill, The University of Manchester, Manchester M13 9PL, UK.
出版信息
Bioresour Technol. 2016 Dec;221:336-343. doi: 10.1016/j.biortech.2016.09.045. Epub 2016 Sep 13.
The inhibitory effect of limonene on polyhydroxybutyrate (PHB) production in Cupriavidus necator H16 was studied. Firstly, results demonstrate the feasibility of using orange juicing waste (OJW) as a substrate for PHB production. An intracellular PHB content of 81.4% (w/w) was attained for a total dry matter concentration of 9.58gL, when the OJW medium was used. Later, a mineral medium designed to mimic the nutrient levels found in the complex medium derived from OJW was used to study the effect of limonene on the production of PHB. Results showed a drop in specific growth rate (μ) of more than 50% when the initial limonene concentration was 2% (v/v) compared to the limonene free medium. This work highlights the importance of a limonene recovery stage prior to fermentation, to maintain levels below 1% (v/v) in the medium, adding value to the OJW and enhancing the fermentation process productivity.
研究了柠檬烯对铜绿假单胞菌 H16 聚羟基丁酸酯（PHB）生产的抑制作用。首先，结果表明使用橙汁榨汁废物（OJW）作为 PHB 生产的底物是可行的。当使用 OJW 培养基时，总干物质浓度为 9.58gL 时，可获得 81.4%（w/w）的细胞内 PHB 含量。后来，使用设计用于模拟源自 OJW 的复杂培养基中发现的营养水平的矿物培养基来研究柠檬烯对 PHB 生产的影响。结果表明，与无柠檬烯培养基相比，当初始柠檬烯浓度为 2%（v/v）时，比生长速率（μ）下降超过 50%。这项工作强调了在发酵前进行柠檬烯回收阶段的重要性，以将培养基中的浓度保持在 1%（v/v）以下，为 OJW 增加价值并提高发酵过程的生产力。
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