Mastboom W J, Hendriks T, de Boer H H
Department of General Surgery, St. Radboud University Hospital, Nijmegen, The Netherlands.
Br J Surg. 1989 Aug;76(8):797-801. doi: 10.1002/bjs.1800760811.
The changes in collagen, measured as hydroxyproline, concentration around both ileal and colonic anastomoses in germ-free and control rats have been investigated and compared with each other. The germ-free rats were raised, operated on and maintained under completely pyrogen-free conditions. Animals were killed 2, 3 or 7 days after operation. There was a significant reduction in the lowering of hydroxyproline concentrations around the colonic anastomosis of germ-free rats compared with control rats both in the anastomosis at days 2 and 3 (P = 0.04 and P = 0.006 respectively) and in the proximal segment at day 3 (P = 0.03). In ileal anastomoses, significant differences between control and germ-free rats were only found at 7 days. Here, the increase in hydroxyproline concentration observed in control rats was significantly reduced in germ-free rats. These data are taken to support the hypothesis that bacteria affect colonic anastomotic healing by contributing to postoperative collagen degradation.
已对无菌大鼠和对照大鼠回肠及结肠吻合口周围以羟脯氨酸衡量的胶原蛋白浓度变化进行了研究，并相互比较。无菌大鼠在完全无热原的条件下饲养、手术和维持。术后2、3或7天处死动物。与对照大鼠相比，无菌大鼠结肠吻合口周围羟脯氨酸浓度降低在吻合口第2天和第3天（分别为P = 0.04和P = 0.006）以及第3天近端段（P = 0.03）均有显著降低。在回肠吻合口中，对照大鼠和无菌大鼠之间仅在第7天发现显著差异。在此，无菌大鼠中对照大鼠观察到的羟脯氨酸浓度增加显著降低。这些数据支持以下假设：细菌通过促进术后胶原蛋白降解影响结肠吻合口愈合。
