Xie Linyan, Liu Qiao, Shao Changshun, Su Xuantao
Opt Express. 2016 Sep 19;24(19):21700-7. doi: 10.1364/OE.24.021700.
Two-dimensional (2D) light scattering patterns of single microspheres, normal granulocytes and leukemic cells are obtained by label-free static cytometry. Statistical results of experimental 2D light scattering patterns obtained from standard microspheres with a mean diameter of 4.19 μm agree well with theoretical simulations. High accuracy rates (greater than 92%) for label-free differentiation of normal granulocytes and leukemic cells, both the acute and chronic leukemic cells, are achieved by analyzing the 2D light scattering patterns. Our label-free static cytometry is promising for leukemia screening in clinics.
通过无标记静态细胞术获得单个微球、正常粒细胞和白血病细胞的二维（2D）光散射模式。从平均直径为4.19μm的标准微球获得的实验二维光散射模式的统计结果与理论模拟结果吻合良好。通过分析二维光散射模式，对正常粒细胞和白血病细胞（包括急性和慢性白血病细胞）进行无标记区分的准确率很高（大于92%）。我们的无标记静态细胞术在临床白血病筛查方面具有广阔前景。
