Differentiating single cervical cells by mitochondrial fluorescence imaging and deep learning-based label-free light scattering with multi-modal static cytometry.

Cervical cancer is a high-risk disease that threatens women's health globally. In this study, we developed the multi-modal static cytometry that adopted different features to classify the typical human cervical epithelial cells (H8) and cervical cancer cells (HeLa). With the light-sheet static cytometry, we obtain brightfield (BF) images, fluorescence (FL) images and two-dimensional (2D) light scattering (LS) patterns of single cervical cells. Three feature extraction methods are used to extract multi-modal features based on different data characteristics. Analysis and classification of morphological and textural features demonstrate the potential of intracellular mitochondria in cervical cancer cell classification. The deep learning method is used to automatically extract deep features of label-free LS patterns, and an accuracy of 76.16% for the classification of the above two kinds of cervical cells is obtained, which is higher than the other two single modes (BF and FL). Our multi-modal static cytometry uses a variety of feature extraction and analysis methods to provide the mitochondria as promising internal biomarkers for cervical cancer diagnosis, and to show the promise of label-free, automatic classification of early cervical cancer with deep learning-based 2D light scattering.