Department of Chemical Sciences, Tezpur University, Assam, 784028, India.
Department of Molecular Biology and Biotechnology, Tezpur University, Assam, 784028, India.
Int J Biol Macromol. 2016 Dec;93(Pt A):1121-1132. doi: 10.1016/j.ijbiomac.2016.09.043. Epub 2016 Sep 21.
Aminated starch coated iron oxide magnetic nanoparticles loaded with curcumin were synthesized via coprecipitation technique. The nanoparticles were crosslinked by using three different crosslinkers: glutaraldehyde, genipin and citric acid and the effect of crosslinking on different properties of the nanoparticles was evaluated. Characterisation of the nanoparticles was done with FTIR (Fourier Transform Infrared spectroscopy) and XRD (X-Ray Diffraction). Magnetic property study using VSM (Vibrating Sample Magnetometer) showed their superparamagnetic nature. Morphology of the nanoparticles was studied by SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy). Zeta potential values showed that crosslinking imparted stability to the system. Crosslinking also enhanced drug loading and encapsulation efficiency of the system. Swelling and in vitro studies of the nanoparticles showed that the release of drug was dependent on time, crosslinker nature, crosslinker concentration and pH of the medium. The aminated starch coated nanoparticles also showed good mucoadhesive character. The cell viability assessment by MTT study revealed their compatibility with human lymphocytes cells and their considerable cell growth inhibiting properties with MCF7 and HepG2 cells. The nanoparticles showed good internalization in HepG2 cells along with considerable ROS formation.
用共沉淀法合成了载姜黄素的胺化淀粉包覆的氧化铁磁性纳米粒子。通过使用三种不同的交联剂：戊二醛、京尼平、柠檬酸对纳米粒子进行交联，并评估交联对纳米粒子不同性质的影响。用傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）对纳米粒子进行了表征。用振动样品磁强计（VSM）进行磁性研究表明它们具有超顺磁性。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究了纳米粒子的形态。Zeta 电位值表明交联赋予了系统稳定性。交联还提高了系统的药物负载和包封效率。纳米粒子的溶胀和体外研究表明，药物的释放取决于时间、交联剂的性质、交联剂浓度和介质的 pH 值。胺化淀粉包覆的纳米粒子还表现出良好的粘膜粘附特性。通过 MTT 研究评估细胞活力，揭示了它们与人淋巴细胞的相容性以及对 MCF7 和 HepG2 细胞的相当大的细胞生长抑制特性。纳米粒子在 HepG2 细胞中表现出良好的内化，并伴随着相当大的 ROS 形成。
