Raziq Naila, Saeed Muhammad, Ali Muhammad Shaiq, Zafar Salman, Shahid Muhammad, Lateef Mehreen
a Department of Pharmacy , Sarhad University of Science and Information Technology , Peshawar , Pakistan.
b Department of Pharmacy , University of Peshawar , Peshawar , Pakistan.
Nat Prod Res. 2017 Jun;31(11):1251-1257. doi: 10.1080/14786419.2016.1236098. Epub 2016 Sep 27.
Phytochemical investigation of Ranunculus muricatus L. (Ranunculaceae) led to the isolation of a new metabolite named as ranuncoside from the ethyl acetate fraction of the plant. Structure of the novel compound was elucidated through detailed spectroscopic analyses, using UV, IR, H, C NMR and 2D NMR in combination with EIMS and HR EI-MS techniques. The compound was evaluated for antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Its inhibitory potential was tested against lipoxygenase and xanthine oxidase enzymes. Ranuncoside potently scavenged the DPPH free radicals (IC = 56.7 ± 0.43 μM) and strongly inhibited the activities of lipoxygenase (IC = 63.9 ± 0.17 μM) and xanthine oxidase (IC = 43.3 ± 0.22 μM).
对毛茛科植物刺果毛茛（Ranunculus muricatus L.）进行植物化学研究，从该植物的乙酸乙酯部位分离出一种名为毛茛糖苷的新代谢产物。通过详细的光谱分析，结合紫外光谱（UV）、红外光谱（IR）、氢核磁共振（H NMR）、碳核磁共振（C NMR）和二维核磁共振（2D NMR）以及电子轰击质谱（EIMS）和高分辨电子轰击质谱（HR EI-MS）技术，阐明了该新型化合物的结构。使用1,1-二苯基-2-苦基肼基（DPPH）自由基清除法对该化合物的抗氧化活性进行了评估。测试了其对脂氧合酶和黄嘌呤氧化酶的抑制潜力。毛茛糖苷能有效清除DPPH自由基（IC₅₀ = 56.7 ± 0.43 μM），并强烈抑制脂氧合酶（IC₅₀ = 63.9 ± 0.17 μM）和黄嘌呤氧化酶（IC₅₀ = 43.3 ± 0.22 μM）的活性。
