一种来自毛茛科石龙芮的新型糖苷类抗氧化剂具有抑制脂氧合酶和黄嘌呤氧化酶的特性。
A new glycosidic antioxidant from Ranunculus muricatus L. (Ranunculaceae) exhibited lipoxygenasae and xanthine oxidase inhibition properties.
作者信息
Raziq Naila, Saeed Muhammad, Ali Muhammad Shaiq, Zafar Salman, Shahid Muhammad, Lateef Mehreen
机构信息
a Department of Pharmacy , Sarhad University of Science and Information Technology , Peshawar , Pakistan.
b Department of Pharmacy , University of Peshawar , Peshawar , Pakistan.
出版信息
Nat Prod Res. 2017 Jun;31(11):1251-1257. doi: 10.1080/14786419.2016.1236098. Epub 2016 Sep 27.
Phytochemical investigation of Ranunculus muricatus L. (Ranunculaceae) led to the isolation of a new metabolite named as ranuncoside from the ethyl acetate fraction of the plant. Structure of the novel compound was elucidated through detailed spectroscopic analyses, using UV, IR, H, C NMR and 2D NMR in combination with EIMS and HR EI-MS techniques. The compound was evaluated for antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Its inhibitory potential was tested against lipoxygenase and xanthine oxidase enzymes. Ranuncoside potently scavenged the DPPH free radicals (IC = 56.7 ± 0.43 μM) and strongly inhibited the activities of lipoxygenase (IC = 63.9 ± 0.17 μM) and xanthine oxidase (IC = 43.3 ± 0.22 μM).
对毛茛科植物刺果毛茛（Ranunculus muricatus L.）进行植物化学研究，从该植物的乙酸乙酯部位分离出一种名为毛茛糖苷的新代谢产物。通过详细的光谱分析，结合紫外光谱（UV）、红外光谱（IR）、氢核磁共振（H NMR）、碳核磁共振（C NMR）和二维核磁共振（2D NMR）以及电子轰击质谱（EIMS）和高分辨电子轰击质谱（HR EI-MS）技术，阐明了该新型化合物的结构。使用1,1-二苯基-2-苦基肼基（DPPH）自由基清除法对该化合物的抗氧化活性进行了评估。测试了其对脂氧合酶和黄嘌呤氧化酶的抑制潜力。毛茛糖苷能有效清除DPPH自由基（IC₅₀ = 56.7 ± 0.43 μM），并强烈抑制脂氧合酶（IC₅₀ = 63.9 ± 0.17 μM）和黄嘌呤氧化酶（IC₅₀ = 43.3 ± 0.22 μM）的活性。
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