Dai Ping-Li, Jia Hui-Ru, Jack Cameron J, Geng Li-Li, Liu Feng, Hou Chun-Sheng, Diao Qing-Yun, Ellis James D
Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China (
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China (
J Econ Entomol. 2016 Dec 1;109(6):2259-2263. doi: 10.1093/jee/tow204.
The cry1Ie gene may be a good candidate for the development of Bt maize because over-expression of Cry1Ie is highly toxic to Lepidopteran pests such as Heliothis armigera Hübner and Ostrinia furnacalis Guenée. The Bt cry1Ie gene also has no cross resistance with other insecticidal proteins such as Cry1Ab, Cry1Ac, Cry1Ah, or Cry1F. Chinese honey bees (Apis cerana cerana) are potentially exposed to insect-resistant genetically modified (IRGM) crops expressing Cry1Ie toxin via the collection of IRGM crop pollen. In this study, we tested whether Chinese honey bee workers are negatively affected by sugar syrup containing 20, 200, or 20,000 ng/ml Cry1Ie toxin and 48 ng/ml imidacloprid under controlled laboratory conditions. Our results demonstrated that the Cry1Ie toxin does not adversely impact survival and pollen consumption of Chinese honey bees. However, imidacloprid decreases Chinese honey bee survival and the total pollen consumption on the 5th, 6th, and 18th d of exposure. The described bioassay is suitable to assess the effects of GM expressed toxins against honey bee.
cry1Ie基因可能是培育转Bt基因玉米的一个良好候选基因，因为Cry1Ie的过表达对棉铃虫和亚洲玉米螟等鳞翅目害虫具有高毒性。Bt cry1Ie基因与其他杀虫蛋白如Cry1Ab、Cry1Ac、Cry1Ah或Cry1F也没有交叉抗性。中华蜜蜂可能会通过采集转抗虫基因作物的花粉接触到表达Cry1Ie毒素的抗虫转基因（IRGM）作物。在本研究中，我们在可控的实验室条件下测试了中华蜜蜂工蜂是否会受到含有20、200或20000 ng/ml Cry1Ie毒素以及48 ng/ml吡虫啉的糖浆的负面影响。我们的结果表明，Cry1Ie毒素不会对中华蜜蜂的存活和花粉消耗产生不利影响。然而，吡虫啉会降低中华蜜蜂的存活率以及在接触的第5、6和18天的总花粉消耗量。所描述的生物测定法适用于评估转基因表达毒素对蜜蜂的影响。
