Kaartinen M, Solin M L, Mäkelä O
Department of Bacteriology and Immunology, University of Helsinki, Finland.
EMBO J. 1989 Jun;8(6):1743-8. doi: 10.1002/j.1460-2075.1989.tb03567.x.
A VH gene (Ox1) has a major role in the early antibody response of several mouse strains to hapten phenyloxazolone (phOx). Antibodies that are coded by this gene are positive for idiotype 495. Idiotype-positive monoclonal antibodies originating from the early primary response of nine strains were partially sequenced (mRNA). All 21 antibodies were coded by this gene, most of them also by one VL gene, VKOx1(H3). Very few somatic mutations were found, and the germ-line sequence of the two genes in several strains can be predicted. Four 'alleles' of the VHOx1 gene have 99-99.7% sequence homology to each other. One allele was found in Igh allotype j strains CBA and C3H, another in allotype c strains DBA/2 and RF, the third in allotype f strain CE and the fourth in BALB/c, 129, A/J and RIII mice (allotypes a, e or g). The VKOx1(H3) gene has the same sequence in eight strains. RF mice do not use this gene for the anti-phOx response. Our data suggest that antibody responses are inherited to a considerable extent and that immunoglobulin V genes are as stable as other genes in evolution.
一种VH基因（Ox1）在几种小鼠品系对半抗原苯恶唑酮（phOx）的早期抗体反应中起主要作用。由该基因编码的抗体对独特型495呈阳性。对来自9个品系早期初次反应的独特型阳性单克隆抗体进行了部分测序（mRNA）。所有21种抗体均由该基因编码，其中大多数也由一个VL基因VKOx1（H3）编码。发现的体细胞突变极少，并且可以预测几种品系中这两个基因的种系序列。VHOx1基因的四个“等位基因”彼此具有99 - 99.7%的序列同源性。在Igh同种异型j品系CBA和C3H中发现了一个等位基因，在同种异型c品系DBA/2和RF中发现了另一个，在同种异型f品系CE中发现了第三个，在BALB/c、129、A/J和RIII小鼠（同种异型a、e或g）中发现了第四个。VKOx1（H3）基因在8个品系中具有相同的序列。RF小鼠在抗phOx反应中不使用该基因。我们的数据表明，抗体反应在很大程度上是可遗传的，并且免疫球蛋白V基因在进化中与其他基因一样稳定。
