Bos N A, Bun J C, Popma S H, Cebra E R, Deenen G J, van der Cammen M J, Kroese F G, Cebra J J
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Infect Immun. 1996 Feb;64(2):616-23. doi: 10.1128/iai.64.2.616-623.1996.
We transferred peritoneal cells from BALB/c mice into C.B17 scid/scid mice. Six to eight months after injection, only cells with the B1 phenotype were retained in the spleens and peritoneal cavities of these mice. The lamina propria of the intestine contained many peritoneal, donor-derived, immunoglobulin A (IgA)-producing cells. The mesenteric lymph nodes of these mice were found to be a major site of proliferation and generation of IgA plasmablasts. We established eight IgA-producing hybridomas from the mesenteric lymph nodes of such mice, and all the hybridomas reacted with different but partially overlapping fecal bacterial populations. Cloning and sequencing of the VH genes of these hybridomas showed that two hybridomas utilized germ line-encoded VH genes while the VH genes of the six hybridomas showed somatic mutations, some of which are indicative of an antigen-driven selection process.
我们将BALB/c小鼠的腹膜细胞移植到C.B17 scid/scid小鼠体内。注射后6至8个月，这些小鼠的脾脏和腹膜腔中仅保留了具有B1表型的细胞。肠道固有层含有许多腹膜来源的、产生免疫球蛋白A（IgA）的供体细胞。这些小鼠的肠系膜淋巴结是IgA浆母细胞增殖和产生的主要部位。我们从这些小鼠的肠系膜淋巴结中建立了8个产生IgA的杂交瘤，所有杂交瘤都与不同但部分重叠的粪便细菌群体发生反应。对这些杂交瘤的VH基因进行克隆和测序表明，两个杂交瘤利用种系编码的VH基因，而六个杂交瘤的VH基因显示出体细胞突变，其中一些表明存在抗原驱动的选择过程。
