Jung Moon Hee, Jung Sang-Myung, Shin Hwa Sung
Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
Sci Rep. 2016 Sep 27;6:33889. doi: 10.1038/srep33889.
Artificial skin or skin equivalents have been used for clinical purpose to skin graft and as substitutes for animal experiments. The culture of cell lines such as HaCaT has the potential to produce large amounts of artificial skin at a low cost. However, there is a limit to keratinization due to the restriction of differentiation in HaCaT. In this study, a culture device that mimics the in vivo keratinization mechanism, co-stimulated by air-exposure and mechanical stimulation, was developed to construct skin equivalents. The device can reconstruct the epidermal morphology, including the cornified layer, similar to its formation in vivo. Under the condition, epidermis was differentiated in the spinous and granular layers. Formation of the stratum corneum is consistent with the mRNA and protein expressions of differentiation markers. The device is the first of its kind to combine air-exposure with mechanical stress to co-stimulate keratinization, which can facilitate the economically viable production of HaCaT-based artificial skin substitutes.
人工皮肤或皮肤替代物已被用于临床皮肤移植以及替代动物实验。诸如HaCaT等细胞系的培养有潜力以低成本生产大量人工皮肤。然而，由于HaCaT中分化的限制，角质化存在局限性。在本研究中，开发了一种模拟体内角质化机制的培养装置，该装置通过空气暴露和机械刺激共同刺激，以构建皮肤替代物。该装置能够重建包括角质层在内的表皮形态，类似于其在体内的形成过程。在此条件下，表皮在棘层和颗粒层发生分化。角质层的形成与分化标志物的mRNA和蛋白质表达一致。该装置是首个将空气暴露与机械应力相结合以共同刺激角质化的同类装置，这有助于以经济可行的方式生产基于HaCaT的人工皮肤替代物。
