Ferencsik M, Garay K, Mihály A, Csillik B
Department of Anatomy, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Exp Brain Res. 1989;76(2):409-16. doi: 10.1007/BF00247898.
ATP, the energy source for axoplasmic transport, is indispensable for the transport of nerve growth factor (NGF). NGF regulates the regeneration of central processes of the primary sensory neurons, by means of transganglionic regulatory mechanisms. This central regeneration was investigated with the help of the histochemical detection of fluoride-resistant acid phosphatase (FRAP), one of the marker enzymes of the primary nociceptive neurons. Transganglionic degenerative atrophy (TDA) of the central terminals of primary sensory neurons was induced with sciatic nerve crush. Dynamics of central regeneration was studied in rats treated with a cobalt-ATP complex, and with commercially available Na-ATP, respectively, by means of histochemical detection of the restitution of FRAP activity in the Rolando substance. Disappearance of FRAP activity was complete on the 6th postoperative day in the medial two-thirds of the upper dorsal horn in segments L2-L6. The regeneration (i.e. replenishment of FRAP activity) began on the 14th day and was complete by the 31st day in animals treated with cobalt-ATP, while in the animals treated with Na-ATP the replenishment of FRAP activity began on the 20th day and was complete only by the 60th day. It is concluded that the cobalt-ATP-complex significantly enhances central regeneration.
三磷酸腺苷（ATP）作为轴浆运输的能量来源，对神经生长因子（NGF）的运输不可或缺。NGF通过跨神经节调节机制来调控初级感觉神经元中枢突的再生。借助对耐氟酸性磷酸酶（FRAP）进行组织化学检测来研究这种中枢再生，FRAP是初级伤害性神经元的标记酶之一。通过坐骨神经挤压诱导初级感觉神经元中枢终末的跨神经节变性萎缩（TDA）。分别采用钴 - ATP复合物和市售的Na - ATP处理大鼠，借助对脊髓后角FRAP活性恢复情况进行组织化学检测，研究中枢再生的动力学过程。术后第6天，L2 - L6节段上背角内侧三分之二区域的FRAP活性完全消失。在用钴 - ATP处理的动物中，再生（即FRAP活性的恢复）在第14天开始，并在第31天完成；而在用Na - ATP处理的动物中，FRAP活性的恢复在第20天开始，直到第60天才完成。结论是钴 - ATP复合物能显著促进中枢再生。
