Kong Xiangjie, Qian Xiaoqiang, Duan Liujian, Liu Hailong, Zhu Yingjian, Qi Jun
1 Department of Urology, Xinhua Hospital, Shanghai Jiaotong University , Shanghai, China .
2 Department of Urology, Ruijin Hospital, Shanghai Jiaotong University , Shanghai, China .
DNA Cell Biol. 2016 Dec;35(12):828-835. doi: 10.1089/dna.2015.3186. Epub 2016 Sep 27.
Prostate cancer (PCa) is one of the most prevalent malignant tumors. microRNAs (miRNAs) play an important role in cancer initiation, progression, and metastasis, and their roles in PCa are becoming more apparent. In this study, we found that microRNA-372 (miR-372) is downregulated in human PCa and inhibits the proliferation activity, migration, and invasion of DU145 cells. Subsequently, p65 is confirmed as a target of miR-372, and knockdown of p65 expression similarly resulted in decreased proliferation activity, migration, and invasion. CDK8, MMP-9, and prostate-specific antigen were involved in both these processes. Taken together, our results show evidence that miR-372 may function as a tumor suppressor gene by regulating p65 in PCa and may provide a strategy for blocking PCa metastasis.
前列腺癌（PCa）是最常见的恶性肿瘤之一。微小RNA（miRNA）在癌症的发生、发展和转移中起重要作用，其在PCa中的作用也日益明显。在本研究中，我们发现微小RNA - 372（miR - 372）在人类PCa中表达下调，并抑制DU145细胞的增殖活性、迁移和侵袭。随后，p65被确认为miR - 372的靶标，敲低p65表达同样导致增殖活性、迁移和侵袭降低。细胞周期蛋白依赖性激酶8（CDK8）、基质金属蛋白酶-9（MMP - 9）和前列腺特异性抗原参与了这两个过程。综上所述，我们的结果表明，miR - 372可能通过调节PCa中的p65发挥肿瘤抑制基因的作用，并可能为阻断PCa转移提供一种策略。
