Yokoi T, Sagisaka K
Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.
Forensic Sci Int. 1989 Apr-May;41(1-2):117-24. doi: 10.1016/0379-0738(89)90243-0.
A recombinant DNA probe hybridizing specifically to human repeat DNA sequence (pHY10) of which about 3000 copies are present on the Y chromosome was used for sex determination of degraded DNA samples of blood stains. Human blood stains of male and female origin were readily differentiated with the pHY10 DNA probe. This radioactive technique enabled reliable and sensitive sex determination from blood or dried blood stains greater than 20 years old. Less than 1 microliter of blood or 1 piece of 0.5 cm length thread of blood stain from cotton fabric was sufficient for the test using dot blot hybridization. Compared with the radioactive labeling method, the photobiotin labeling method showed one thirtieth to one fiftieth lower sensitivity and presented some problems which are expected to be resolvable.
一种与人类重复DNA序列（pHY10）特异性杂交的重组DNA探针被用于血迹降解DNA样本的性别鉴定，该序列在Y染色体上约有3000个拷贝。使用pHY10 DNA探针可以很容易地区分男性和女性来源的人类血迹。这种放射性技术能够对20多年前的血液或干血迹进行可靠且灵敏的性别鉴定。使用斑点印迹杂交法进行检测时，少于1微升的血液或从棉织物上取下的长度为0.5厘米的一根血迹丝线就足够了。与放射性标记法相比，光生物素标记法的灵敏度低三十分之一到五十分之一，且存在一些有望解决的问题。
