Kobayashi R, Hibino T, Miyata H, Iizuka N, Nonomura S, Matsuzawa S
Nihon Hoigaku Zasshi. 1989 Feb;43(1):58-63.
Bloodstains collected from criminal spots were submitted to the nonisotopic Southern hybridization (NISH) method for sex identification (Kobayashi et al., J. Forens. Sci., 33, 613-620, 1988). A RNA probe, transcripted from a Y-chromosome specific DNA sequence (Nakahori et al., Nucl. Aci. Res., 14, 7569-7580), was used for this series of investigations. Among the 95 bloodstain specimens studied, in which the sex of each was previously known, 54 (88.5%) of 61 male stains were appropriately determined to be male by the NISH method, and 28 (82.4%) of 34 female bloodstain specimens were estimated to be female. The sex of the 13 remaining stain specimens were unable to be determined by this method, but no specimen was erroneously misidentified. With regard to 12 specimens, in which the sex of each was unknown at the time of the NISH test, 5 were determined as being male and 3 were estimated as being female, though the sex of the remaining 4 specimens could not be determined. The causes in the stains that made determination impossible were analyzed using patterns of ethidium bromide fluorescence of electrophoresed gel and/or NISH, and it was concluded that this could be due to an insufficient quantity of extracted DNA (12 cases), the resistance against a restriction enzyme (EcoRI) (3 cases), and the depolymerization of the Y-chromosome specific fragment (2 cases).
从犯罪现场采集的血迹采用非同位素Southern杂交（NISH）方法进行性别鉴定（小林等人，《法医学杂志》，33卷，613 - 620页，1988年）。使用从Y染色体特异性DNA序列转录而来的RNA探针（中堀等人，《核酸研究》，14卷，7569 - 7580页）进行这一系列调查。在所研究的95份血迹标本中，每份标本的性别事先已知，61份男性血迹中有54份（88.5%）通过NISH方法被正确判定为男性，34份女性血迹标本中有28份（82.4%）被判定为女性。其余13份血迹标本的性别无法通过该方法确定，但没有标本被错误鉴定。对于12份在进行NISH检测时性别未知的标本，5份被判定为男性，3份被判定为女性，其余4份标本的性别无法确定。利用电泳凝胶和/或NISH的溴化乙锭荧光模式分析了导致无法确定的血迹原因，得出结论认为这可能是由于提取的DNA量不足（12例）、对限制性内切酶（EcoRI）有抗性（3例）以及Y染色体特异性片段解聚（2例）。
